1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. KAT5 Protein, Human (His)

KAT5 Protein, Human (His)

Cat. No.: HY-P71707
COA Handling Instructions

The KAT5 protein is the catalytic subunit of the NuA4 histone acetyltransferase complex and activates gene transcription through histone acetylation. KAT5 Protein, Human (His) is the recombinant human-derived KAT5 protein, expressed by E. coli , with N-6*His labeled tag. The total length of KAT5 Protein, Human (His) is 511 a.a., with molecular weight of ~62.4 kDa.

For research use only. We do not sell to patients.

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Description

The KAT5 protein is the catalytic subunit of the NuA4 histone acetyltransferase complex and activates gene transcription through histone acetylation. KAT5 Protein, Human (His) is the recombinant human-derived KAT5 protein, expressed by E. coli , with N-6*His labeled tag. The total length of KAT5 Protein, Human (His) is 511 a.a., with molecular weight of ~62.4 kDa.

Background

KAT5, the catalytic subunit of the NuA4 histone acetyltransferase complex, is a key participant in the transcriptional activation of specific genes through the acetylation of nucleosomal histones H2A and H4. This acetylation modifies nucleosome-DNA interactions and facilitates the interaction of modified histones with other proteins, positively regulating transcription. The NuA4 complex, essential for various cellular processes such as growth induction, growth arrest, apoptosis, and DNA repair, plays a direct role in repairing DNA double-strand breaks by inhibiting TP53BP1 binding to chromatin. Additionally, KAT5 acetylates non-histone proteins involved in diverse pathways, including DNA repair, circadian regulation, autophagy, innate antiviral responses, and lipid metabolism. It acts as a crucial regulator of chromosome segregation and kinetochore-microtubule attachment during mitosis, catalyzing acetylation or crotonylation of target proteins to ensure accurate chromosome segregation and spindle positioning. KAT5's multifaceted roles make it a central player in cellular homeostasis and genome maintenance.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

E. coli

Tag

N-6*His

Accession

Q92993 (E3-W513)

Gene ID
Molecular Construction
N-term
6*His
KAT5 (E3-W513)
Accession # Q92993
C-term
Synonyms
60kDa Tat interactive protein; cPLA2 interacting protein; ESA1; Histone acetyltransferase HTATIP; HTATIP1; K(lysine) acetyltransferase 5; PLIP; Tat interacting protein, 60kDa; TIP; Tip60
AA Sequence

EVGEIIEGCRLPVLRRNQDNEDEWPLAEILSVKDISGRKLFYVHYIDFNKRLDEWVTHERLDLKKIQFPKKEAKTPTKNGLPGSRPGSPEREVPASAQASGKTLPIPVQITLRFNLPKEREAIPGGEPDQPLSSSSCLQPNHRSTKRKVEVVSPATPVPSETAPASVFPQNGAARRAVAAQPGRKRKSNCLGTDEDSQDSSDGIPSAPRMTGSLVSDRSHDDIVTRMKNIECIELGRHRLKPWYFSPYPQELTTLPVLYLCEFCLKYGRSLKCLQRHLTKCDLRHPPGNEIYRKGTISFFEIDGRKNKSYSQNLCLLAKCFLDHKTLYYDTDPFLFYVMTEYDCKGFHIVGYFSKEKESTEDYNVACILTLPPYQRRGYGKLLIEFSYELSKVEGKTGTPEKPLSDLGLLSYRSYWSQTILEILMGLKSESGERPQITINEISEITSIKKEDVISTLQYLNLINYYKGQYILTLSEDIVDGHERAMLKRLLRIDSKCLHFTPKDWSKRGKW

Molecular Weight

Approximately 62.4 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm sterile filtered 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

KAT5 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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KAT5 Protein, Human (His)
Cat. No.:
HY-P71707
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