KAT5 Protein, Human (His)
Based on 1 publication(s) in Google Scholar
The KAT5 protein is the catalytic subunit of the NuA4 histone acetyltransferase complex and activates gene transcription through histone acetylation. KAT5 Protein, Human (His) is the recombinant human-derived KAT5 protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
The KAT5 protein is the catalytic subunit of the NuA4 histone acetyltransferase complex and activates gene transcription through histone acetylation. KAT5 Protein, Human (His) is the recombinant human-derived KAT5 protein, expressed by E. coli , with N-6*His labeled tag.
KAT5, the catalytic subunit of the NuA4 histone acetyltransferase complex, is a key participant in the transcriptional activation of specific genes through the acetylation of nucleosomal histones H2A and H4. This acetylation modifies nucleosome-DNA interactions and facilitates the interaction of modified histones with other proteins, positively regulating transcription. The NuA4 complex, essential for various cellular processes such as growth induction, growth arrest, apoptosis, and DNA repair, plays a direct role in repairing DNA double-strand breaks by inhibiting TP53BP1 binding to chromatin. Additionally, KAT5 acetylates non-histone proteins involved in diverse pathways, including DNA repair, circadian regulation, autophagy, innate antiviral responses, and lipid metabolism. It acts as a crucial regulator of chromosome segregation and kinetochore-microtubule attachment during mitosis, catalyzing acetylation or crotonylation of target proteins to ensure accurate chromosome segregation and spindle positioning. KAT5's multifaceted roles make it a central player in cellular homeostasis and genome maintenance.
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Publications (1)
-
Journal Impact Factor
-
Most Recent
Technical Parameters
-
Species Human
-
Source E. coli
-
Tag N-6*His
-
Accession
Q92993 (E3-W513)
-
Molecular Construction
-
N-term
-
6*His
-
KAT5 (E3-W513)
Accession # Q92993 -
C-term
-
-
Protein Length
Partial
-
Synonyms
KAT5; 60 KDa Tat-Interactive Protein; Prev. HTATIP; Protein Lactyltransferase KAT5; TIP60; K(Lysine) Acetyltransferase 5; HTATIP1; CPLA(2)-Interacting Protein; CPLA2; K-Acetyltransferase 5; PLIP; ZC2HC5; ESA1; HIV-1 Tat Interactive Protein; Protein 2-Hydr
-
AA Sequence
EVGEIIEGCRLPVLRRNQDNEDEWPLAEILSVKDISGRKLFYVHYIDFNKRLDEWVTHERLDLKKIQFPKKEAKTPTKNGLPGSRPGSPEREVPASAQASGKTLPIPVQITLRFNLPKEREAIPGGEPDQPLSSSSCLQPNHRSTKRKVEVVSPATPVPSETAPASVFPQNGAARRAVAAQPGRKRKSNCLGTDEDSQDSSDGIPSAPRMTGSLVSDRSHDDIVTRMKNIECIELGRHRLKPWYFSPYPQELTTLPVLYLCEFCLKYGRSLKCLQRHLTKCDLRHPPGNEIYRKGTISFFEIDGRKNKSYSQNLCLLAKCFLDHKTLYYDTDPFLFYVMTEYDCKGFHIVGYFSKEKESTEDYNVACILTLPPYQRRGYGKLLIEFSYELSKVEGKTGTPEKPLSDLGLLSYRSYWSQTILEILMGLKSESGERPQITINEISEITSIKKEDVISTLQYLNLINYYKGQYILTLSEDIVDGHERAMLKRLLRIDSKCLHFTPKDWSKRGKW
-
Predicted Molecular Mass
65.3 kDa
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
2.Lyophilized from a 0.2 μm sterile filtered PBS, 6% trehalose, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (263 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
