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Kirrel1/NEPH1 Protein, Human (HEK293, Fc)

The Kirrel1/NEPH1 protein is essential for the normal function of the glomerular filtration barrier in the kidney, maintaining stable podocyte structure through finger-like foot processes and gap septal cell-cell junctions. As a signaling molecule, it requires TEC kinase to fully activate the transcription factor AP-1. Kirrel1/NEPH1 Protein, Human (HEK293, Fc) is the recombinant human-derived Kirrel1/NEPH1 protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Kirrel1/NEPH1 Protein, Human (HEK293, Fc) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The Kirrel1/NEPH1 protein is essential for the normal function of the glomerular filtration barrier in the kidney, maintaining stable podocyte structure through finger-like foot processes and gap septal cell-cell junctions. As a signaling molecule, it requires TEC kinase to fully activate the transcription factor AP-1. Kirrel1/NEPH1 Protein, Human (HEK293, Fc) is the recombinant human-derived Kirrel1/NEPH1 protein, expressed by HEK293 , with C-hFc labeled tag.

Background

Kirrel1/NEPH1 Protein is crucial for the proper function of the glomerular filtration barrier in the kidneys. It plays a vital role in maintaining a stable podocyte architecture by facilitating the interdigitating foot processes connected by specialized cell-cell junctions called the slit diaphragm. This protein acts as a signaling molecule and requires the presence of TEC kinases to fully activate the transcription factor AP-1. Kirrel1/NEPH1 interacts with TJP1/ZO-1 and NPHS2/podocin, and its interaction with NPHS1/nephrin relies on Kirrel1/NEPH1 glycosylation. It forms homodimers through its Ig-like domains and, when tyrosine-phosphorylated, it interacts with GRB2.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-hFc
  • Accession

    Q96J84-1 (Q17-L493)

  • Gene ID
    55243  [NCBI]
  • Molecular Construction
    • N-term
    • Kirrel1/NEPH1 (Q17-L493)
      Accession # Q96J84-1
    • hFc
    • C-term
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    KIRREL1; Kin Of Irregular Chiasm-Like Protein 1; Prev. KIRREL; Kin Of IRRE Like (Drosophila); NEPH1; Kin Of IRRE Like; Kin Of IRRE-Like Protein 1; Nephrin Related; Nephrin-Like Protein 1; NPHS23; Kirre Like Nephrin Family Adhesion Molecule 1; CDNA FLJ5636

  • AA Sequence

    QGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVL

  • Predicted Molecular Mass

    78.8 kDa

  • Molecular Weight

    Approximately 96 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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