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Klotho Protein, Human (CHO, His)

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Based on 1 publication(s) in Google Scholar

Klotho Protein exhibits weak glycosidase activity against glucuronidated steroids and is inactive in vivo due to the lack of essential active site Glu residues. It regulates calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Klotho Protein, Human (CHO, His) is the recombinant human-derived Klotho protein, expressed by CHO , with C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: CHO
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Klotho Protein, Human (CHO, His)
  • Experimental Validation Results for Klotho Protein, Human (CHO, His)
  • Klotho Protein, Human (CHO, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Klotho Protein exhibits weak glycosidase activity against glucuronidated steroids and is inactive in vivo due to the lack of essential active site Glu residues. It regulates calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Klotho Protein, Human (CHO, His) is the recombinant human-derived Klotho protein, expressed by CHO , with C-10*His labeled tag.

Background

The Klotho protein exhibits weak glycosidase activity towards glucuronylated steroids, but its in vivo inactivity as a glycosidase is suggested by the absence of essential active site Glu residues at positions 239 and 872. Additionally, Klotho may play a role in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. As an essential factor for the specific interaction between FGF23 and FGFR1, Klotho contributes to crucial signaling pathways. Furthermore, the Klotho peptide, generated by cleaving the membrane-bound isoform, is proposed to function as an anti-aging circulating hormone. This hormone has the potential to extend lifespan by inhibiting insulin/IGF1 signaling, presenting Klotho as a multifunctional protein with implications in both metabolic regulation and the aging process.

Verified Bioactivity

1.The EC50 as determined by the dose-dependent stimulation of the proliferation of murine NIH/3T3 is less than 1 μg/mL.
2.Immobilized rHuFGF-23 at 2.0 μg/mL (100 μL/well) can bind Klotho Protein, Human with EC50 of 0.64-1.25 μg/mL.

Technical Parameters

  • Species Human
  • Source CHO
  • Tag C-10*His
  • Accession

    Q9UEF7 (E34-S981)

  • Gene ID
    9365  [NCBI]
  • Molecular Construction
    • N-term
    • Klotho (E34-S981)
      Accession # Q9UEF7
    • 10*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    KL; Alpha-Klotho; Klotho; HFTC3; KLA

  • AA Sequence

    EPGDGAQTWARFSRPPAPEAAGLFQGTFPDGFLWAVGSAAYQTEGGWQQHGKGASIWDTFTHHPLAPPGDSRNASLPLGAPSPLQPATGDVASDSYNNVFRDTEALRELGVTHYRFSISWARVLPNGSAGVPNREGLRYYRRLLERLRELGVQPVVTLYHWDLPQRLQDAYGGWANRALADHFRDYAELCFRHFGGQVKYWITIDNPYVVAWHGYATGRLAPGIRGSPRLGYLVAHNLLLAHAKVWHLYNTSFRPTQGGQVSIALSSHWINPRRMTDHSIKECQKSLDFVLGWFAKPVFIDGDYPESMKNNLSSILPDFTESEKKFIKGTADFFALCFGPTLSFQLLDPHMKFRQLESPNLRQLLSWIDLEFNHPQIFIVENGWFVSGTTKRDDAKYMYYLKKFIMETLKAIKLDGVDVIGYTAWSLMDGFEWHRGYSIRRGLFYVDFLSQDKMLLPKSSALFYQKLIEKNGFPPLPENQPLEGTFPCDFAWGVVDNYIQVDTTLSQFTDLNVYLWDVHHSKRLIKVDGVVTKKRKSYCVDFAAIQPQIALLQEMHVTHFRFSLDWALILPLGNQSQVNHTILQYYRCMASELVRVNITPVVALWQPMAPNQGLPRLLARQGAWENPYTALAFAEYARLCFQELGHHVKLWITMNEPYTRNMTYSAGHNLLKAHALAWHVYNEKFRHAQNGKISIALQADWIEPACPFSQKDKEVAERVLEFDIGWLAEPIFGSGDYPWVMRDWLNQRNNFLLPYFTEDEKKLIQGTFDFLALSHYTTILVDSEKEDPIKYNDYLEVQEMTDITWLNSPSQVAVVPWGLRKVLNWLKFKYGDLPMYIISNGIDDGLHAEDDQLRVYYMQNYINEALKAHILDGINLCGYFAYSFNDRTAPRFGLYRYAADQFEPKASMKHYRKIIDSNGFPGPETLERFCPEEFTVCTECSFFHTRKS

  • Molecular Weight

    Approximately 110-130 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.2 μm filtered solution of PBS, 50% Glycerol, pH 7.4.
2.Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 5% Trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<0.1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cell proliferation assay using NIH-3T3 cells. The ED50 for this effect is 304.8 ng/mL.

  • Klotho Protein, Human (CHO, His) Product Image
    1/16
  • Experimental Validation Results for Klotho Protein, Human (CHO, His)
  • Experimental Validation Results for Klotho Protein, Human (CHO, His)
  • Klotho Protein, Human (CHO, His) Product Image