KSR2 Protein, Human (sf9, GST)

KSR2 Protein, Human (sf9, GST) is the recombinant human-derived KSR2, expressed by Sf9 insect cells , with GST labeled tag. ,

KSR2 is a location-regulated scaffold protein that connects MEK to RAF. It exhibits very low protein kinase activity and can phosphorylate multiple Ser and Thr residues of MAP2K1 with minimal efficiency in vitro. Acting as a MAP2K1/MEK1-dependent allosteric activator of BRAF, KSR2 dimerizes with BRAF upon binding to MAP2K1/MEK1, promoting BRAF-mediated phosphorylation of MAP2K1/MEK1. Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). KSR2 also negatively regulates signaling by blocking MAP3K8 kinase activity and MAP3K8-mediated pathways, while inhibiting MAP3K3-driven activation of ERK, JNK, and NF-kappa-B pathways, including MAP3K3-mediated interleukin-8 production.

Human

Sf9 insect cells

GST

Q6VAB6-1 (R554-L950)

283455

Partial

RQQKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNPILEGNPLLQIEVEPTSENEEVHDEAEESEDDFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL

≥ 90%, as determined by reducing SDS-PAGE.