KSR2 Protein, Human (sf9, GST)
KSR2 Protein, Human (sf9, GST) is the recombinant human-derived KSR2, expressed by Sf9 insect cells , with GST labeled tag. ,
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
KSR2 Protein, Human (sf9, GST) is the recombinant human-derived KSR2, expressed by Sf9 insect cells , with GST labeled tag. ,
Background
KSR2 is a location-regulated scaffold protein that connects MEK to RAF. It exhibits very low protein kinase activity and can phosphorylate multiple Ser and Thr residues of MAP2K1 with minimal efficiency in vitro. Acting as a MAP2K1/MEK1-dependent allosteric activator of BRAF, KSR2 dimerizes with BRAF upon binding to MAP2K1/MEK1, promoting BRAF-mediated phosphorylation of MAP2K1/MEK1. Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). KSR2 also negatively regulates signaling by blocking MAP3K8 kinase activity and MAP3K8-mediated pathways, while inhibiting MAP3K3-driven activation of ERK, JNK, and NF-kappa-B pathways, including MAP3K3-mediated interleukin-8 production.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag GST
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Accession
Q6VAB6-1 (R554-L950)
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Gene ID283455
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Protein Length
Partial
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Synonyms
KSR2; FLJ25965; Kinase Suppressor Of Ras 2; HKSR2
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AA Sequence
RQQKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNPILEGNPLLQIEVEPTSENEEVHDEAEESEDDFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL
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Molecular Weight
Approximately 72 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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