L-arabinose isomerase Protein, Bacillus subtilis (His)

L-arabinose isomerase is a key enzyme in carbohydrate metabolism and plays a key role in catalyzing the conversion of L-arabinose to L-ribulose. This enzymatic activity is part of a broader biochemical pathway involved in the interconversion of sugars. L-arabinose isomerase Protein, Bacillus subtilis (His) is the recombinant L-arabinose isomerase protein, expressed by E. coli , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Others
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

L-arabinose isomerase is a key enzyme in carbohydrate metabolism and plays a key role in catalyzing the conversion of L-arabinose to L-ribulose. This enzymatic activity is part of a broader biochemical pathway involved in the interconversion of sugars. L-arabinose isomerase Protein, Bacillus subtilis (His) is the recombinant L-arabinose isomerase protein, expressed by E. coli , with N-6*His labeled tag.

Background

L-arabinose isomerase is an enzyme that plays a crucial role in carbohydrate metabolism by catalyzing the conversion of L-arabinose to L-ribulose. This enzymatic transformation is significant in the interconversion of different sugar isomers, contributing to the metabolic pathways involved in the utilization of arabinose. By facilitating the conversion of L-arabinose to L-ribulose, L-arabinose isomerase is essential for the subsequent utilization of these sugar derivatives in various cellular processes. The enzyme's ability to mediate this specific isomerization reaction highlights its importance in the broader context of carbohydrate metabolism and the intricate network of biochemical pathways within the cell.

Technical Parameters

  • Species Others
  • Source E. coli
  • Tag N-6*His
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • 6*His
    • araA (M1-K496)
      Accession # P94523
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    BSU28800; araA; EC:5.3.1.4; L-arabinose isomerase

  • AA Sequence

    MLQTKDYEFWFVTGSQHLYGEETLELVDQHAKSICEGLSGISSRYKITHKPVVTSPETIRELLREAEYSETCAGIITWMHTFSPAKMWIEGLSSYQKPLMHLHTQYNRDIPWGTIDMDFMNSNQSAHGDREYGYINSRMGLSRKVIAGYWDDEEVKKEMSQWMDTAAALNESRHIKVARFGDNMRHVAVTDGDKVGAHIQFGWQVDGYGIGDLVEVMDRITDDEVDTLYAEYDRLYVISEETKRDEAKVASIKEQAKIELGLTAFLEQGGYTAFTTSFEVLHGMKQLPGLAVQRLMEKGYGFAGEGDWKTAALVRMMKIMAKGKRTSFMEDYTYHFEPGNEMILGSHMLEVCPTVALDQPKIEVHSLSIGGKEDPARLVFNGISGSAIQASIVDIGGRFRLVLNEVNGQEIEKDMPNLPVARVLWKPEPSLKTAAEAWILAGGAHHTCLSYELTAEQMLDWAEMAGIESVLISRDTTIHKLKHELKWNEALYRLQK

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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=
Mass (in vial) Mass (in vial)
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Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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