Latexin Protein, Mouse (His)

Latexin protein, possessing notable properties, highly potently inhibits CPA1, CPA2, and CPA4 enzymes in a hardly reversible, non-competitive manner. Its potential role in inflammation regulation is suggested. Latexin Protein, Mouse (His) is the recombinant mouse-derived Latexin protein, expressed by E. coli , with N-His labeled tag. The total length of Latexin Protein, Mouse (His) is 221 a.a., with molecular weight of ~28-32 kDa.

Latexin, a protein with notable properties, acts as a highly potent inhibitor of CPA1, CPA2, and CPA4 enzymes in a hardly reversible, non-competitive manner. Additionally, it is suggested to potentially play a role in the regulation of inflammation.

Measured by its ability to inhibit carboxypeptidase A1 cleavage of the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). The IC₅₀ value is 0.555 nM, as measured under the described conditions.

Mouse

E. coli

N-His

P70202 (E2-E222)

17035  [NCBI]

Approximately 28-32 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0, 10% Glycerol or 50 mM HEPES, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.