Latexin Protein, Mouse (His)
Based on 1 Customer Validation
Latexin protein, possessing notable properties, highly potently inhibits CPA1, CPA2, and CPA4 enzymes in a hardly reversible, non-competitive manner.Its potential role in inflammation regulation is suggested.Latexin Protein, Mouse (His) is the recombinant mouse-derived Latexin protein, expressed by E.coli , with N-His labeled tag.
- Species: Mouse
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Latexin protein, possessing notable properties, highly potently inhibits CPA1, CPA2, and CPA4 enzymes in a hardly reversible, non-competitive manner.Its potential role in inflammation regulation is suggested.Latexin Protein, Mouse (His) is the recombinant mouse-derived Latexin protein, expressed by E.coli , with N-His labeled tag.
Latexin, a protein with notable properties, acts as a highly potent inhibitor of CPA1, CPA2, and CPA4 enzymes in a hardly reversible, non-competitive manner. Additionally, it is suggested to potentially play a role in the regulation of inflammation.
Measured by its ability to inhibit carboxypeptidase A1 cleavage of the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). The IC50 value is 0.555 nM, as measured under the described conditions. (Activation description: The proenzyme needs to be activated by Trypsin for an activated form.)
Technical Parameters
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Species Mouse
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Source E. coli
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Tag N-His
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Accession
P70202 (E2-E222)
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Molecular Construction
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N-term
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His
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Latexin (E2-E222)
Accession # P70202 -
C-term
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Protein Length
Partial
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Synonyms
LXN; ECI; Latexin; TCI; Endogenous Carboxypeptidase Inhibitor; Protein MUM; Tissue Carboxypeptidase Inhibitor; MUM
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AA Sequence
EIPPTHYAASRAASVAENCINYQQGTPHKLFLVQTVQQASKEDIPGRGHKYHLKFSVEEIIQKQVTVNCTAEVLYPQMGQGSAPEVNFTFEGEIGKNPDEEDNTFYQSLMSLKRPLEAQDIPDNFGNVSPQMKPVQHLAWVACGYVMWQNSTEDTWYKMLKIQTVKQVQRNDDFIELDYTILLHDIASQEIIPWQMQVLWHPQYGTKVKHNSRLPKEGQAE
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Molecular Weight
Approximately 32 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of 50 mM HEPES, 300 mM NaCl, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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