LDLR Protein, Rabbit (HEK293, Fc)
LDLR Protein binds LDL, transporting cholesterol via endocytosis. Clustering in clathrin-coated pits initiates internalization. Interactions include DAB2 (via PID domain) through the NPXY motif, hindered by tyrosine phosphorylation. LDLR also interacts with LDLRAP1 (via PID domain) through the NPXY motif, ARRB1, SNX17, and the full-length immature PCSK9 through the C-terminus. LDLR Protein, Rabbit (HEK293, Fc) is the recombinant Rabbit-derived LDLR protein, expressed by HEK293 , with C-hFc labeled tag. The total length of LDLR Protein, Rabbit (HEK293, Fc) is 757 a.a., with molecular weight of 120-140 kDa.
- Species: Rabbit
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
LDLR Protein binds LDL, transporting cholesterol via endocytosis. Clustering in clathrin-coated pits initiates internalization. Interactions include DAB2 (via PID domain) through the NPXY motif, hindered by tyrosine phosphorylation. LDLR also interacts with LDLRAP1 (via PID domain) through the NPXY motif, ARRB1, SNX17, and the full-length immature PCSK9 through the C-terminus. LDLR Protein, Rabbit (HEK293, Fc) is the recombinant Rabbit-derived LDLR protein, expressed by HEK293 , with C-hFc labeled tag. The total length of LDLR Protein, Rabbit (HEK293, Fc) is 757 a.a., with molecular weight of 120-140 kDa.
Background
LDLR protein functions by binding to LDL, the primary lipoprotein responsible for carrying cholesterol in the bloodstream, and facilitating its uptake into cells through endocytosis. For internalization to occur, the receptor-ligand complexes must cluster within clathrin-coated pits. It engages in interactions with various proteins, including DAB2 (via PID domain) through the NPXY motif, although tyrosine phosphorylation of the NPXY motif can hinder this interaction. LDLR also interacts with LDLRAP1 (via PID domain) via the NPXY motif, as well as with ARRB1 and SNX17. Additionally, it interacts with the full-length immature form of PCSK9, specifically through the C-terminus.
Technical Parameters
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Species Rabbit
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Source HEK293
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Tag C-hFc
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Accession
P20063 (A9-G765)
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Gene ID100009086
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Molecular Construction
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N-term
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LDLR (A9-G765)
Accession # P20063 -
hFc
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C-term
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Protein Length
Partial Extracellular Domain
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Synonyms
LDLR; LDL Receptor; Low Density Lipoprotein Receptor; Low-Density Lipoprotein Receptor Class A Domain-Containing Protein 3; Low-Density Lipoprotein Receptor; Familial Hypercholesterolemia; LDLCQ2; Low Density Lipoprotein
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AA Sequence
AAGDKCGRNEFQCRNGKCISYKWVCDGSSECQDGSDEWEQTCMSLTCKSDDFSCGGRLNRCIPGHWKCDGQQDCEDGSDELGCAPKTCSQDEFRCAEGACISRLFACDGEPDCPDGSDEASCAPSTCGPAHFRCNSSSCVPALWACDGEPDCDDGSDEWPARCGARPSPQPGRGPCSRHEFHCGSGECVHASWRCDGDADCRDGSDERDCAAATCRPDEFQCSDGTCIHGSRQCDQQQDCGDMSDEVGCVNVTLCEGPDKFKCHSGECISLDKVCNSARDCQDWSDEPIKECATNECMRGNGGCSHTCFDLRIGHECHCPKGYRLVDQRRCEDINECEDPDICSQLCVNLAGSYKCECRAGFQLDPHSQACKAVDSIAYLFFTNRHEVRKMTLDRSEYTSLIANLKNVVALDAEVASNRIYWSDLSQRKIYSAQIDGAHGFPAYDTVISSDLQAPDGLAVDWIHGHIYWTDSVLGTVSVADTRGFRRKTLFRQEGSKPRAIVVDPAHGFMYWTDWGVPAKIEKGGLNGVDVYSLVTEDIQWPNGITLDLSSGRLYWVDSKLHSISSIDVNGGNRKTVLEDEQRLAHPFSLAIFEDKVFWTDVINEAIFSANRLTGSDVHLVAENLLSPEDIVLFHNLTQPRGVNWCEKTALPNGGCQYLCLPAPQINSHSPKFTCACPDGTLLAADMRSCRTEADVILSTQRASTAARPQLTGSPAGTTQEPLTEPTLSTLETATTSQQALHNADGRGSEGTPRSVG
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Predicted Molecular Mass
109.2 kDa
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Molecular Weight
Approximately 120-140 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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