Legumain Protein, Pig (416a.a, HEK293, His)

Legumain proteins can slowly cleave aspartyl bonds, especially under acidic conditions. Legumain Protein, Pig (416a.a, HEK293, His) is the recombinant pig-derived Legumain protein, expressed by HEK293 , with N-His labeled tag. The total length of Legumain Protein, Pig (416a.a, HEK293, His) is 416 a.a., with molecular weight of ~52.5 kDa.

Legumain protein exhibits a strict specificity for the hydrolysis of asparaginyl bonds. Additionally, it demonstrates the ability to cleave aspartyl bonds slowly, particularly in acidic conditions, further expanding its enzymatic versatility. Functionally, Legumain is integral to the processing of proteins for MHC class II antigen presentation within the lysosomal/endosomal system. It also plays a crucial role in MHC class I antigen presentation in cross-presenting dendritic cells by facilitating the cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol, as indicated by recent research findings. Moreover, Legumain is essential for normal lysosomal protein degradation in renal proximal tubules and is required for the degradation of internalized EGFR, highlighting its importance in cellular processes and the regulation of cell proliferation.

Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methylcoumarin (Z-AAN-AMC). The specific activity is 2781.650 pmoL/min/µg, as measured under the described conditions.

Pig

HEK293

N-6*His

XP_001927117 (V18-Y433)

100154477  [NCBI]

VPVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIVHRNGIPDEQIIVMMYDDIANSEDNPTPGIVINRPNGSDVYKGVLKDYTGEDVTPQNFLAVLRGDAEAVKGKGSGKVLKSGPRDHVFVYFTDHGATGILVFPNEDLHVKDLNETIHYMYKHRMYQKMVFYIEACESGSMMNHLPPNIDVYATTAANPRESSYACYYDEARATYLGDWYSVNWMEDSDSEDLTRETLHKQYQLVKSHTNTSHVMQYGNKSISAMKLVQFQGLKHKASSPISLPPVQHLDLTPSPEVPLTIMKRKLTRTNDLQESRRLVAEIDRHLQARNVIEKSVRKIVSLTVGSDAEVDRLLSQRDPLTAHECYQEAVLHFRTHCFNWHSPTYEYALRHLYVLANLCENSYPIASIKLSMDKVCLGYY

Approximately 57 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCL, 50 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.