Legumain Protein, Pig (HEK293, His)
Based on 1 Customer Validation
Legumain proteins can slowly cleave aspartyl bonds, especially under acidic conditions. Legumain Protein, Pig (HEK293, His) is the recombinant pig-derived Legumain protein, expressed by HEK293 , with N-His labeled tag.
- Species: Pig
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Legumain proteins can slowly cleave aspartyl bonds, especially under acidic conditions. Legumain Protein, Pig (HEK293, His) is the recombinant pig-derived Legumain protein, expressed by HEK293 , with N-His labeled tag.
Background
Legumain protein exhibits a strict specificity for the hydrolysis of asparaginyl bonds. Additionally, it demonstrates the ability to cleave aspartyl bonds slowly, particularly in acidic conditions, further expanding its enzymatic versatility. Functionally, Legumain is integral to the processing of proteins for MHC class II antigen presentation within the lysosomal/endosomal system. It also plays a crucial role in MHC class I antigen presentation in cross-presenting dendritic cells by facilitating the cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol, as indicated by recent research findings. Moreover, Legumain is essential for normal lysosomal protein degradation in renal proximal tubules and is required for the degradation of internalized EGFR, highlighting its importance in cellular processes and the regulation of cell proliferation.
Verified Bioactivity
Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methylcoumarin (Z-AAN-AMC). The specific activity is 2781.650 pmoL/min/µg, as measured under the described conditions. (Activation description: The proenzyme needs to be activated in acid buffer for an activated form.)
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Pig
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Source HEK293
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Tag N-6*His
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Accession
XP_001927117 (V18-Y433)
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Molecular Construction
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N-term
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His
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Legumain (V18-Y433)
Accession # XP_001927117 -
C-term
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Protein Length
Full Length of Legumain Chain
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Synonyms
LGMN; LGMN1; Prev. PRSC1; AEP; Asparaginyl Endopeptidase; Cysteine Protease 1; Protease, Cysteine 1; Legumain; Protease, Cysteine, 1 (Legumain)
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AA Sequence
VPVDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIVHRNGIPDEQIIVMMYDDIANSEDNPTPGIVINRPNGSDVYKGVLKDYTGEDVTPQNFLAVLRGDAEAVKGKGSGKVLKSGPRDHVFVYFTDHGATGILVFPNEDLHVKDLNETIHYMYKHRMYQKMVFYIEACESGSMMNHLPPNIDVYATTAANPRESSYACYYDEARATYLGDWYSVNWMEDSDSEDLTRETLHKQYQLVKSHTNTSHVMQYGNKSISAMKLVQFQGLKHKASSPISLPPVQHLDLTPSPEVPLTIMKRKLTRTNDLQESRRLVAEIDRHLQARNVIEKSVRKIVSLTVGSDAEVDRLLSQRDPLTAHECYQEAVLHFRTHCFNWHSPTYEYALRHLYVLANLCENSYPIASIKLSMDKVCLGYY
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Molecular Weight
Approximately 53 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 50 mM NaCl, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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