Serpin B1 Protein, Pig (His, Myc)

The leukocyte elastase inhibitor/SERPINB1 protein is an important neutrophil serine protease inhibitor that regulates innate immune responses by protecting cells from cytoplasmic proteases during stress. It inhibits various neutrophil proteases, including elastase and chymotrypsin, and blocks GZMH activity intracellularly. Serpin B1 Protein, Pig (His, Myc) is the recombinant pig-derived Leukocyte elastase inhibitor/SERPINB1 protein, expressed by E. coli , with N-His, C-Myc labeled tag.

The Leukocyte elastase inhibitor/SERPINB1 protein, a neutrophil serine protease inhibitor, is pivotal in regulating the innate immune response, inflammation, and cellular homeostasis. Its primary function is to shield the cell from proteases released in the cytoplasm during stress or infection, preventing potential harm caused by the potent enzymes released from granules. SERPINB1 effectively regulates neutrophil proteases, including elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Furthermore, it acts as a potent intracellular inhibitor of GZMH, directly impeding its proteolytic activity. During inflammation, SERPINB1 curtails the activity of inflammatory caspases (CASP1, CASP4, and CASP5) by inhibiting their caspase-recruitment domain (CARD) oligomerization and enzymatic activation. When secreted, SERPINB1 promotes beta-cell proliferation through its protease inhibitory function. It may undergo cleavage, resulting in a loss of anti-protease activity and the emergence of an endonuclease activity, though no specific catalytic site has been identified.

Pig

E. coli

N-His;C-Myc

P80229 (M1-P378)

100155145  [NCBI]

Full Length

MEQLSAANTRFALDLFRALNESNPAGNIFISPFSISSALAMILLGTRGNTEAQMSKALHFDTVKDIHSRFQSLNADINKCGASYILKLANRLFGEKTYHFLPEFLASTQKTYGAELASVDFLRASEEARKAINEWVKEQTEGKIPELLASGVVDSATKLVLVNAIYFKGSWQEKFMTEATKDAPFRLNKKDSKTVKMMYQKKKFPFGYIKELKCRVLELPYQGKDLSMVILLPDSIEDESTGLRKIEQHLTLEKLNEWTKPDNLELLEVNVHLPRFRLEESYDLNAPLARLGVQDLFGSRADLTGMSEARDLFISKVVHKAFVEVNEEGTEAAAATAGIAVFAMLMPEEDFIADHPFIFFIRHNPSSNILFLGRLSSP

Approximately 50.0 kDa

• 
  ≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.