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  2. Enzymes & Regulators
  3. Protease Inhibitors
  4. Serpin (Protease Inhibitor)
  5. Leukocyte Elastase Inhibitor
  6. Leukocyte elastase inhibitor/SERPINB1 Protein, Pig (His, myc)

Leukocyte elastase inhibitor/SERPINB1 Protein, Pig (His, myc)

Cat. No.: HY-P72289
COA Handling Instructions

The leukocyte elastase inhibitor/SERPINB1 protein is an important neutrophil serine protease inhibitor that regulates innate immune responses by protecting cells from cytoplasmic proteases during stress. It inhibits various neutrophil proteases, including elastase and chymotrypsin, and blocks GZMH activity intracellularly. Leukocyte elastase inhibitor/SERPINB1 Protein, Pig (His, myc) is the recombinant pig-derived Leukocyte elastase inhibitor/SERPINB1 protein, expressed by E. coli , with N-His, C-Myc labeled tag. The total length of Leukocyte elastase inhibitor/SERPINB1 Protein, Pig (His, myc) is 378 a.a., with molecular weight of ~50.0 kDa.

For research use only. We do not sell to patients.

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Description

The leukocyte elastase inhibitor/SERPINB1 protein is an important neutrophil serine protease inhibitor that regulates innate immune responses by protecting cells from cytoplasmic proteases during stress. It inhibits various neutrophil proteases, including elastase and chymotrypsin, and blocks GZMH activity intracellularly. Leukocyte elastase inhibitor/SERPINB1 Protein, Pig (His, myc) is the recombinant pig-derived Leukocyte elastase inhibitor/SERPINB1 protein, expressed by E. coli , with N-His, C-Myc labeled tag. The total length of Leukocyte elastase inhibitor/SERPINB1 Protein, Pig (His, myc) is 378 a.a., with molecular weight of ~50.0 kDa.

Background

The Leukocyte elastase inhibitor/SERPINB1 protein, a neutrophil serine protease inhibitor, is pivotal in regulating the innate immune response, inflammation, and cellular homeostasis. Its primary function is to shield the cell from proteases released in the cytoplasm during stress or infection, preventing potential harm caused by the potent enzymes released from granules. SERPINB1 effectively regulates neutrophil proteases, including elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Furthermore, it acts as a potent intracellular inhibitor of GZMH, directly impeding its proteolytic activity. During inflammation, SERPINB1 curtails the activity of inflammatory caspases (CASP1, CASP4, and CASP5) by inhibiting their caspase-recruitment domain (CARD) oligomerization and enzymatic activation. When secreted, SERPINB1 promotes beta-cell proliferation through its protease inhibitory function. It may undergo cleavage, resulting in a loss of anti-protease activity and the emergence of an endonuclease activity, though no specific catalytic site has been identified.

Species

Pig

Source

E. coli

Tag

N-His;C-Myc

Accession

P80229 (M1-P378)

Gene ID

100155145  [NCBI]

Synonyms
SERPINB1; ELANH2Leukocyte elastase inhibitor; LNPI; Serpin B1
AA Sequence

MEQLSAANTRFALDLFRALNESNPAGNIFISPFSISSALAMILLGTRGNTEAQMSKALHFDTVKDIHSRFQSLNADINKCGASYILKLANRLFGEKTYHFLPEFLASTQKTYGAELASVDFLRASEEARKAINEWVKEQTEGKIPELLASGVVDSATKLVLVNAIYFKGSWQEKFMTEATKDAPFRLNKKDSKTVKMMYQKKKFPFGYIKELKCRVLELPYQGKDLSMVILLPDSIEDESTGLRKIEQHLTLEKLNEWTKPDNLELLEVNVHLPRFRLEESYDLNAPLARLGVQDLFGSRADLTGMSEARDLFISKVVHKAFVEVNEEGTEAAAATAGIAVFAMLMPEEDFIADHPFIFFIRHNPSSNILFLGRLSSP

Molecular Weight

Approximately 50.0 kDa

Purity
  • Greater than 90% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Leukocyte elastase inhibitor/SERPINB1 Protein, Pig (His, myc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Leukocyte elastase inhibitor/SERPINB1 Protein, Pig (His, myc)
Cat. No.:
HY-P72289
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