lipoxygenase Protein, Pseudomonas aeruginosa (Flag, His, CL7)
The lipoxygenase protein catalyzes the oxygen-mediated conversion of linoleic acid to 9HPOD and spontaneously decomposes it into 9HOD. It converts linolenic acid into 13HPOD, which breaks down into 13HOD. lipoxygenase Protein, Human (Flag, His, CL7) is the recombinant human-derived lipoxygenase, expressed by E. coli, with CL7, His, Flag labeled tag.
- Species: Others
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The lipoxygenase protein catalyzes the oxygen-mediated conversion of linoleic acid to 9HPOD and spontaneously decomposes it into 9HOD. It converts linolenic acid into 13HPOD, which breaks down into 13HOD. lipoxygenase Protein, Human (Flag, His, CL7) is the recombinant human-derived lipoxygenase, expressed by E. coli, with CL7, His, Flag labeled tag.
Background
In the presence of oxygen, the lipoxygenase protein catalyzes the conversion of linoleate into (9S)-hydroperoxy-10,12-octadecenoate (9HPOD), with subsequent spontaneous decomposition into the corresponding 9-hydroxy-10,12-octadecenoate (9HOD). It also transforms linolenate into its hydroperoxy derivative, 13-hydroperoxy-9,11-octadecenoate (13HPOD), which spontaneously decomposes to the corresponding 13-hydroxy-9,11-octadecenoate (13HOD). Additionally, the enzyme exhibits activity on oleate, albeit to a lesser extent, converting it into (10S)-hydroperoxy-8E-octadecenoate, which undergoes spontaneous decomposition to form the corresponding 10-hydroxy-8E-octadecenoate. Notably, lipoxygenase demonstrates minimal activity on arachidonate. This enzymatic versatility highlights its role in mediating diverse fatty acid oxygenation reactions, contributing to the intricate metabolic pathways associated with lipid signaling and inflammation.
Technical Parameters
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Species Others
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Source E. coli
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Tag CL7;His;Flag
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Accession
Q8RNT4 (M1-I685)
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Gene ID/
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Protein Length
Full Length
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Synonyms
LOX; Lysyl Oxidase Homolog; Lysyl Oxidase; AAT10; Protein-Lysine 6-Oxidase
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AA Sequence
MKRRSVLLSGVALSGTALANDSIFFSPLKYLGAEQQRSIDASRSLLDNLIPPSLPQYDNLAGKLARRAVLTSKKLVYVWTENFANVKGVPMARSVPLGELPNVDWLLKTAGVIVELIVNFVASLPASAAAQFERIAAGLSGDLEAARQVHEALLEEAKNDPAAAGSLLLRFTELQTRVIALLTRVGLLVDDILKSASNLVTQGGQGDGLNRFRAVFGTLRLPEVADSFRDDEAFAYWRVAGPNPLLIRRVDALPANFPLGEEQFRRVMGADDSLLEAAASRRLYLLDYAELGKLAPSGAVDKLLTGTGFAYAPIALFALGKDRAGLLPVAIQCGQDPATHPMFVRPAESESDLYWGWQMAKTVVQVAEENYHEMFVHLAQTHLVSEAFCLATQRTLAPSHPLHVLLAPHFEGTLFINEGAARILLPSAGFIDVMFAAPIQDTQATAGGNRLGFDFYRGMLPESLKARNVDDPAALPDYPYRDDGLLVWNAIRQWAADYVAVYYASDGDVTADVELAAWVGEVIGSGKVAGFRPITGRSQLVEVLTMVIFTASAQHAAVNFPQPSMMTYAPAICAMSAAPAPDSPSGKSEADWLKMMPPTLVALEKVNIYHLLGSVYHGRLGDYRQTGFPYAPVFSDRRVTASGGPLERFQARLKEVEATIRTRNQARRKPYEYLLPSRIPASTNI
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Predicted Molecular Mass
91.3 kDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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