LIV-1/SLC39A6 Protein, Human (HEK293, His)
Based on 1 Customer Validation
LIV-1/SLC39A6 is a zinc influx transporter that regulates zinc homeostasis and induces epithelial-mesenchymal transition (EMT).Used in conjunction with SLC39A10, it can promote cellular zinc absorption and trigger EMT.LIV-1/SLC39A6 Protein, Human (HEK293, His) is the recombinant human-derived LIV-1/SLC39A6 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
LIV-1/SLC39A6 is a zinc influx transporter that regulates zinc homeostasis and induces epithelial-mesenchymal transition (EMT).Used in conjunction with SLC39A10, it can promote cellular zinc absorption and trigger EMT.LIV-1/SLC39A6 Protein, Human (HEK293, His) is the recombinant human-derived LIV-1/SLC39A6 protein, expressed by HEK293 , with C-His labeled tag.
Background
LIV-1/SLC39A6, a zinc-influx transporter, intricately regulates zinc homeostasis and contributes to the induction of epithelial-to-mesenchymal transition (EMT). Functionally, when forming a heterodimer with SLC39A10, this complex mediates cellular zinc uptake, serving as a pivotal trigger for EMT. The SLC39A10-SLC39A6 heterodimer not only controls NCAM1 phosphorylation but also influences its integration into focal adhesion complexes during EMT. The zinc influx facilitated by this heterodimeric complex plays a crucial role in inactivating GSK3B, leading to nuclear accumulation of unphosphorylated SNAI1, which subsequently down-regulates adherence genes like CDH1, thereby promoting loss of cell adherence. Beyond its involvement in EMT, the SLC39A10-SLC39A6 heterodimer plays a fundamental role in initiating mitosis by importing zinc into cells, triggering a pathway that culminates in the onset of mitosis. Additionally, this transporter complex contributes to T-cell receptor signaling regulation and facilitates proper zinc influx for meiotic progression during the oocyte-to-egg transition.
Verified Bioactivity
1.Immobilized Human LIV-1 His at 1 μg/mL (100 μL/well) can bind Anti-LIV-1 antibody Human IgG1. The EC50 for this effect is 0.2-9 ng/mL.
2.Immobilized Human LIV-1 at 2 μg/mL can bind Anti-Human LIV-1 recombinant antibody. The EC50 for this effect is 0.8021-1.744 ng/mL.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-10*His
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Accession
Q13433-1 (F29-W325)
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Molecular Construction
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N-term
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Reg1 (F29-W325)
Accession # Q13433-1 -
10*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
SLC39A6; Solute Carrier Family 39 (Zinc Transporter), Member 6; Solute Carrier Family 39 Member 6; Estrogen-Regulated Protein LIV-1; ZIP6; ZRT/IRT-Like Protein 6; Zrt- And Irt-Like Protein 6; Zinc Transporter 6; Zinc Transporter ZIP6; ZIP-6; LIV-1; LIV1;
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AA Sequence
FPQTTEKISPNWESGINVDLAISTRQYHLQQLFYRYGENNSLSVEGFRKLLQNIGIDKIKRIHIHHDHDHHSDHEHHSDHERHSDHEHHSEHEHHSDHDHHSHHNHAASGKNKRKALCPDHDSDSSGKDPRNSQGKGAHRPEHASGRRNVKDSVSASEVTSTVYNTVSEGTHFLETIETPRPGKLFPKDVSSSTPPSVTSKSRVSRLAGRKTNESVSEPRKGFMYSRNTNENPQECFNASKLLTSHGMGIQVPLNATEFNYLCPAIINQIDARSCLIHTSEKKAEIPPKTYSLQIAW
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Predicted Molecular Mass
35.5 kDa
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Molecular Weight
Approximately 55-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
1.Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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