AMSH-LP Protein, Human
Based on 1 Customer Validation
AMSH-LP is a zinc metalloprotease that selectively cleaves "Lys-63" linked polyubiquitin chains and acts as a positive regulator of the TORC1 signaling pathway. It specifically deubiquitinates SESN2, disrupts its interaction with the GATOR2 complex and inhibits SESN2-mediated regulation of TORC1. AMSH-LP Protein, Human is the recombinant human-derived AMSH-LP protein, expressed by E. coli , with tag free.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
AMSH-LP is a zinc metalloprotease that selectively cleaves "Lys-63" linked polyubiquitin chains and acts as a positive regulator of the TORC1 signaling pathway. It specifically deubiquitinates SESN2, disrupts its interaction with the GATOR2 complex and inhibits SESN2-mediated regulation of TORC1. AMSH-LP Protein, Human is the recombinant human-derived AMSH-LP protein, expressed by E. coli , with tag free.
AMSH-LP (AMSHLP), a zinc metalloprotease, exhibits a selective ability to cleave 'Lys-63'-linked polyubiquitin chains, without affecting 'Lys-48'-linked polyubiquitin chains. Notably, AMSHLP serves as a positive regulator of the TORC1 signaling pathway through its role in the 'Lys-63'-linked deubiquitination of SESN2. This deubiquitination activity impedes the interaction between SESN2 and the GATOR2 complex, thereby contributing to the inhibition of SESN2-mediated TORC1 regulation. These functions underscore AMSHLP's specific involvement in fine-tuning cellular signaling pathways, particularly those associated with TORC1, through precise modulation of 'Lys-63'-linked polyubiquitin chains.
Technical Parameters
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Species Human
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Source E. coli
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Tag Tag Free
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Accession
Q96FJ0 (D2-R436)
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Gene ID57559
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Molecular Construction
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N-term
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LP (D2-R436)
Accession # Q96FJ0 -
C-term
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Protein Length
Partial
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Synonyms
STAMBPL1; AMSH-like protease; AMSH-LP; STAM-binding protein-like 1
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AA Sequence
DQPFTVNSLKKLAAMPDHTDVSLSPEERVRALSKLGCNITISEDITPRRYFRSGVEMERMASVYLEEGNLENAFVLYNKFITLFVEKLPNHRDYQQCAVPEKQDIMKKLKEIAFPRTDELKNDLLKKYNVEYQEYLQSKNKYKAEILKKLEHQRLIEAERKRIAQMRQQQLESEQFLFFEDQLKKQELARGQMRSQQTSGLSEQIDGSALSCFSTHQNNSLLNVFADQPNKSDATNYASHSPPVNRALTPAATLSAVQNLVVEGLRCVVLPEDLCHKFLQLAESNTVRGIETCGILCGKLTHNEFTITHVIVPKQSAGPDYCDMENVEELFNVQDQHDLLTLGWIHTHPTQTAFLSSVDLHTHCSYQLMLPEAIAIVCSPKHKDTGIFRLTNAGMLEVSACKKKGFHPHTKEPRLFSICKHVLVKDIKIIVLDLR
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Predicted Molecular Mass
49.8 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 10 mM HEPES, 150 mM NaCl, 5% glycerol, pH 7.5.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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