LRP-5 Protein, Human (Biotinylated, HEK293, His-Avi)

The LRP-5 protein functions as a coreceptor, cooperating with Frizzled receptors to initiate the canonical Wnt signaling pathway. It is critical for cell fate determination and self-renewal and plays an important role in posterior ectoderm patterning during gastrulation and bone development by affecting osteoblast proliferation and differentiation. LRP-5 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived LRP-5 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of LRP-5 Protein, Human (Biotinylated, HEK293, His-Avi) is 620 a.a., with molecular weight of 70-90 kDa.

LRP-5 protein acts as a pivotal coreceptor, partnering with the frizzled family of seven-transmembrane spanning receptors to transduce signals initiated by Wnt proteins. This activation leads to the canonical Wnt signaling pathway, a critical regulator of cell fate determination and self-renewal during both embryonic development and adult tissue regeneration. LRP-5 is particularly implicated in the posterior patterning of the epiblast during gastrulation and plays a crucial role in bone development by regulating osteoblast proliferation and differentiation. Mechanistically, LRP-5 facilitates the formation of the signaling complex between Wnt ligands, frizzled receptors, and itself, leading to the recruitment of AXIN1. This stabilizes beta-catenin/CTNNB1 and activates TCF/LEF-mediated transcriptional programs. Moreover, LRP-5 serves as a coreceptor for non-Wnt proteins, such as norrin/NDP, with a demonstrated role in retinal vascular development. The interaction with various partners, including FZD8, AXIN1, DKK1, MESD, KREMEN2, CSNK1E, SOST, APCDD1, and CAPRIN2, highlights its intricate involvement in the regulation of diverse cellular processes.

Immobilized Human SOST His at 5 μg/mL (100 μL/well) can bind Biotinylated Human LRP-5 His-Avi with a linear range of 0.078-1.25 μg/mL.

Human

HEK293

C-Avi;C-His

O75197 (E644-Q1263)

4041  [NCBI]

Partial

Biotin

EAFLVFTSRAAIHRISLETNNNDVAIPLTGVKEASALDFDVSNNHIYWTDVSLKTISRAFMNGSSVEHVVEFGLDYPEGMAVDWMGKNLYWADTGTNRIEVARLDGQFRQVLVWRDLDNPRSLALDPTKGYIYWTEWGGKPRIVRAFMDGTNCMTLVDKVGRANDLTIDYADQRLYWTDLDTNMIESSNMLGQERVVIADDLPHPFGLTQYSDYIYWTDWNLHSIERADKTSGRNRTLIQGHLDFVMDILVFHSSRQDGLNDCMHNNGQCGQLCLAIPGGHRCGCASHYTLDPSSRNCSPPTTFLLFSQKSAISRMIPDDQHSPDLILPLHGLRNVKAIDYDPLDKFIYWVDGRQNIKRAKDDGTQPFVLTSLSQGQNPDRQPHDLSIDIYSRTLFWTCEATNTINVHRLSGEAMGVVLRGDRDKPRAIVVNAERGYLYFTNMQDRAAKIERAALDGTEREVLFTTGLIRPVALVVDNTLGKLFWVDADLKRIESCDLSGANRLTLEDANIVQPLGLTILGKHLYWIDRQQQMIERVEKTTGDKRTRIQGRVAHLTGIHAVEEVSLEEFSAHPCARDNGGCSHICIAKGDGTPRCSCPVHLVLLQNLLTCGEPPTCSPDQ

70-90 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally trehalose is added as protectant before lyophilization.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.