LRP-5 Protein, Human (HEK293, mFc)
Based on 1 Customer Validation
The LRP-5 protein functions as a coreceptor, cooperating with Frizzled receptors to initiate the canonical Wnt signaling pathway. It is critical for cell fate determination and self-renewal and plays an important role in posterior ectoderm patterning during gastrulation and bone development by affecting osteoblast proliferation and differentiation. LRP-5 Protein, Human (HEK293, mFc) is the recombinant human-derived LRP-5 protein, expressed by HEK293 , with C-mFc labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The LRP-5 protein functions as a coreceptor, cooperating with Frizzled receptors to initiate the canonical Wnt signaling pathway. It is critical for cell fate determination and self-renewal and plays an important role in posterior ectoderm patterning during gastrulation and bone development by affecting osteoblast proliferation and differentiation. LRP-5 Protein, Human (HEK293, mFc) is the recombinant human-derived LRP-5 protein, expressed by HEK293 , with C-mFc labeled tag.
Background
LRP-5 protein acts as a pivotal coreceptor, partnering with the frizzled family of seven-transmembrane spanning receptors to transduce signals initiated by Wnt proteins. This activation leads to the canonical Wnt signaling pathway, a critical regulator of cell fate determination and self-renewal during both embryonic development and adult tissue regeneration. LRP-5 is particularly implicated in the posterior patterning of the epiblast during gastrulation and plays a crucial role in bone development by regulating osteoblast proliferation and differentiation. Mechanistically, LRP-5 facilitates the formation of the signaling complex between Wnt ligands, frizzled receptors, and itself, leading to the recruitment of AXIN1. This stabilizes beta-catenin/CTNNB1 and activates TCF/LEF-mediated transcriptional programs. Moreover, LRP-5 serves as a coreceptor for non-Wnt proteins, such as norrin/NDP, with a demonstrated role in retinal vascular development. The interaction with various partners, including FZD8, AXIN1, DKK1, MESD, KREMEN2, CSNK1E, SOST, APCDD1, and CAPRIN2, highlights its intricate involvement in the regulation of diverse cellular processes.
Verified Bioactivity
Immobilized Human DKK1, His Tag at 5 μg/mL (100μl/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of <0.15 μg/mL determined by ELISA.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-mFc
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Accession
O75197 (E644-Q1263)
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Molecular Construction
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N-term
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LRP-5 (E644-Q1263)
Accession # O75197 -
mFc
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C-term
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Protein Length
Partial
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Synonyms
LRP5; HBM; Prev. LRP7; Low Density Lipoprotein Receptor-Related Protein 5; Prev. EVR1; LRP-5; Prev. OPPG; LRP-7; LR3; Low Density Lipoprotein Receptor-Related Protein 7; Low-Density Lipoprotein Receptor-Related Protein 5; Low-Density Lipoprotein Receptor-
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AA Sequence
EAFLVFTSRAAIHRISLETNNNDVAIPLTGVKEASALDFDVSNNHIYWTDVSLKTISRAFMNGSSVEHVVEFGLDYPEGMAVDWMGKNLYWADTGTNRIEVARLDGQFRQVLVWRDLDNPRSLALDPTKGYIYWTEWGGKPRIVRAFMDGTNCMTLVDKVGRANDLTIDYADQRLYWTDLDTNMIESSNMLGQERVVIADDLPHPFGLTQYSDYIYWTDWNLHSIERADKTSGRNRTLIQGHLDFVMDILVFHSSRQDGLNDCMHNNGQCGQLCLAIPGGHRCGCASHYTLDPSSRNCSPPTTFLLFSQKSAISRMIPDDQHSPDLILPLHGLRNVKAIDYDPLDKFIYWVDGRQNIKRAKDDGTQPFVLTSLSQGQNPDRQPHDLSIDIYSRTLFWTCEATNTINVHRLSGEAMGVVLRGDRDKPRAIVVNAERGYLYFTNMQDRAAKIERAALDGTEREVLFTTGLIRPVALVVDNTLGKLFWVDADLKRIESCDLSGANRLTLEDANIVQPLGLTILGKHLYWIDRQQQMIERVEKTTGDKRTRIQGRVAHLTGIHAVEEVSLEEFSAHPCARDNGGCSHICIAKGDGTPRCSCPVHLVLLQNLLTCGEPPTCSPDQ
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Predicted Molecular Mass
95.4 kDa
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Molecular Weight
Approximately 95-115 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE or Bis-Tris PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (240 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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