LRPAP1 Protein, Human (HEK293, C-His)

LRPAP1 protein functions as a molecular chaperone for LDL receptor-related proteins, regulating ligand binding throughout the secretory pathway. It transiently interacts with LRP1/alpha-2-macroglobulin receptor chains, reducing ligand binding. LRPAP1 also interacts with LRP2/glycoprotein 330, LDLR, and SORL1, influencing internalization, degradation, or ligand binding modulation. This highlights LRPAP1's multifaceted role in governing the function and trafficking of LDL receptor-related proteins. LRPAP1 Protein, Human (HEK293, C-His) is the recombinant human-derived LRPAP1 protein, expressed by HEK293, with C-6*His labeled tag. The total length of LRPAP1 Protein, Human (HEK293, C-His) is 323 a.a., with molecular weight of ~42 KDa.

LRPAP1 protein serves as a molecular chaperone for LDL receptor-related proteins, contributing to the regulation of their ligand binding activity throughout the secretory pathway. Notably, LRPAP1 engages in transient interactions with the LRP1/alpha-2-macroglobulin receptor heavy and light chains, coinciding with a reduction in ligand binding by the receptor. Additionally, LRPAP1 forms interactions with LRP2/glycoprotein 330, LDLR, and SORL1, with diverse outcomes ranging from internalization and degradation to modulating ligand binding. The intricate network of interactions underscores the multifaceted role of LRPAP1 in orchestrating the function and trafficking of LDL receptor-related proteins.

Immobilized LRPAP1 at 0.5 μg/mL (100 μL/well) can bind Biotinylated VLDLR protein. The ED₅₀ for this effect is 0.5626 nM.

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  Immobilized LRPAP1 at 0.5 μg/mL (100 μL/well) can bind Biotinylated VLDLR protein. The ED₅₀ for this effect is 0.5626 nM.

Human

HEK293

C-6*His

P30533 (Y35-L357)

4043

YSREKNQPKPSPKRESGEEFRMEKLNQLWEKAQRLHLPPVRLAELHADLKIQERDELAWKKLKLDGLDEDGEKEARLIRNLNVILAKYGLDGKKDARQVTSNSLSGTQEDGLDDPRLEKLWHKAKTSGKFSGEELDKLWREFLHHKEKVHEYNVLLETLSRTEEIHENVISPSDLSDIKGSVLHSRHTELKEKLRSINQGLDRLRRVSHQGYSTEAEFEEPRVIDLWDLAQSANLTDKELEAFREELKHFEAKIEKHNHYQKQLEIAHEKLRHAESVGDGERVSRSREKHALLEGRTKELGYTVKKHLQDLSGRISRARHNEL

Approximately 42 kDa.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.