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LRPAP1 Protein, Human (HEK293, C-His)

2 Cited Publications
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Based on 2 publication(s) in Google Scholar

The LRPAP1 protein serves as a molecular chaperone for LDL receptor-related proteins and regulates ligand binding throughout the secretory pathway. It transiently interacts with the LRP1/α-2-macroglobulin receptor chain, reducing ligand binding. LRPAP1 Protein, Human (HEK293, C-His) is the recombinant human-derived LRPAP1 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for LRPAP1 Protein, Human (HEK293, C-His)
  • Experimental Validation Results for LRPAP1 Protein, Human (HEK293, C-His)
  • LRPAP1 Protein, Human (HEK293, C-His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

The LRPAP1 protein serves as a molecular chaperone for LDL receptor-related proteins and regulates ligand binding throughout the secretory pathway. It transiently interacts with the LRP1/α-2-macroglobulin receptor chain, reducing ligand binding. LRPAP1 Protein, Human (HEK293, C-His) is the recombinant human-derived LRPAP1 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

LRPAP1 protein serves as a molecular chaperone for LDL receptor-related proteins, contributing to the regulation of their ligand binding activity throughout the secretory pathway. Notably, LRPAP1 engages in transient interactions with the LRP1/alpha-2-macroglobulin receptor heavy and light chains, coinciding with a reduction in ligand binding by the receptor. Additionally, LRPAP1 forms interactions with LRP2/glycoprotein 330, LDLR, and SORL1, with diverse outcomes ranging from internalization and degradation to modulating ligand binding. The intricate network of interactions underscores the multifaceted role of LRPAP1 in orchestrating the function and trafficking of LDL receptor-related proteins.

Verified Bioactivity

Immobilized LRPAP1 at 0.5 μg/mL (100 μL/well) can bind Biotinylated VLDLR protein (HY-P78049). The KD for this effect is < 0.6 nM.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Immobilized LRPAP1 at 0.5 μg/mL (100 μL/well) can bind Biotinylated VLDLR protein. The ED50 for this effect is 0.5626 nM.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P30533 (Y35-L357)

  • Gene ID
    4043
  • Molecular Construction
    • N-term
    • LRPAP1 (Y35-L357)
      Accession # P30533
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    LRPAP1; Low Density Lipoprotein-Related Protein-Associated Protein 1 (Alpha-2-Macroglobulin Receptor-Associated Protein 1); Prev. A2MRAP; Low Density Lipoprotein Receptor-Related Protein-Associated Protein 1; Prev. RAP; 39 KDa Receptor-Associated Protein;

  • AA Sequence

    YSREKNQPKPSPKRESGEEFRMEKLNQLWEKAQRLHLPPVRLAELHADLKIQERDELAWKKLKLDGLDEDGEKEARLIRNLNVILAKYGLDGKKDARQVTSNSLSGTQEDGLDDPRLEKLWHKAKTSGKFSGEELDKLWREFLHHKEKVHEYNVLLETLSRTEEIHENVISPSDLSDIKGSVLHSRHTELKEKLRSINQGLDRLRRVSHQGYSTEAEFEEPRVIDLWDLAQSANLTDKELEAFREELKHFEAKIEKHNHYQKQLEIAHEKLRHAESVGDGERVSRSREKHALLEGRTKELGYTVKKHLQDLSGRISRARHNEL

  • Predicted Molecular Mass

    38.6 kDa

  • Molecular Weight

    Approximately 37-42 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized LRPAP1 at 0.5 μg/mL (100 μL/well) can bind Biotinylated VLDLR protein. The ED50 for this effect is 0.5626 nM.

  • LRPAP1 Protein, Human (HEK293, C-His) Product Image
    1/16
  • Experimental Validation Results for LRPAP1 Protein, Human (HEK293, C-His)
  • Experimental Validation Results for LRPAP1 Protein, Human (HEK293, C-His)
  • LRPAP1 Protein, Human (HEK293, C-His) Product Image