LRRK2 Protein, Human (Active, G2019S, sf9, GST)
LRRK2 Protein, Human (sf9, GST, G2019S) is the recombinant human-derived LRRK2, expressed by sf9 insect cells , with GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
LRRK2 Protein, Human (sf9, GST, G2019S) is the recombinant human-derived LRRK2, expressed by sf9 insect cells , with GST labeled tag.
Background
LRRK2 is a serine/threonine-protein kinase that phosphorylates a broad range of proteins involved in neuronal plasticity, innate immunity, autophagy, and vesicle trafficking. It serves as a key regulator of RAB GTPases by controlling their GTP/GDP exchange and interaction partners through phosphorylation. LRRK2 phosphorylates RAB3A, RAB3B, RAB3C, RAB3D, RAB5A, RAB5B, RAB5C, RAB8A, RAB8B, RAB10, RAB12, RAB29, RAB35, and RAB43. By phosphorylating RAB8A at Thr-72, it modulates RAB3IP-catalyzed GDP/GTP exchange and inhibits RAB8A binding to GDI1/GDI2. Additionally, LRRK2 promotes primary ciliogenesis via phosphorylation of RAB8A and RAB10, enhancing SHH signaling in the brain. Together with RAB29, it facilitates retromer-dependent retrograde trafficking between lysosomes and the Golgi apparatus. LRRK2 regulates neuronal morphology, synaptic vesicle trafficking, and endoplasmic reticulum exit site (ERES) organization. It positively regulates autophagy through calcium-dependent activation of the CaMKK/AMPK pathway and phosphorylates PRDX3 and APP (promoting AICD production and nuclear translocation). In innate immunity, LRRK2 enhances RIPK2 activation downstream of NOD1/NOD2. Independently of its kinase activity, it inhibits MAPT proteasomal degradation, promoting oligomerization and secretion. Its Roc domain exhibits GTPase activity, modulating LRRK2 kinase function. Recruited by RAB29/RAB7L1 to overloaded lysosomes, LRRK2 stabilizes RAB8A and RAB10 via phosphorylation, facilitating lysosomal content release and suppressing lysosomal enlargement.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag GST
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Accession
Q5S007 (H970-E2527, G2019S)
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Gene ID120892
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Protein Length
Partial
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Synonyms
LRRK2; DKFZp434H2111; Prev. PARK8; Non-Specific Serine/Threonine Protein Kinase; Leucine-Rich Repeat Serine/Threonine-Protein Kinase 2; Parkinson Disease (Autosomal Dominant) 8; Dardarin; Augmented In Rheumatoid Arthritis 17; ROCO2; AURA17; RIPK7; Leucine
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AA Sequence
HSDSISSLASEREYITSLDLSANELRDIDALSQKCCISVHLEHLEKLELHQNALTSFPQQLCETLKSLTHLDLHSNKFTSFPSYLLKMSCIANLDVSRNDIGPSVVLDPTVKCPTLKQFNLSYNQLSFVPENLTDVVEKLEQLILEGNKISGICSPLRLKELKILNLSKNHISSLSENFLEACPKVESFSARMNFLAAMPFLPPSMTILKLSQNKFSCIPEAILNLPHLRSLDMSSNDIQYLPGPAHWKSLNLRELLFSHNQISILDLSEKAYLWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDELHLNFDFKHIGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKTKKSDLGMQSATVGIDVKDWPIQIRDKRKRDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACMSKITKELLNKRGFPAIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQLVVGQLIPDCYVELEKIILSERKNVPIEFPVIDRKRLLQLVRENQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKIMAQILTVKVEGCPKHPKGIISRRDVEKFLSKKRKFPKNYMSQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPYMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNHPESFLKITVPSCRKGCILLGQVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDDLMKKAEEGDLLVNPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYSIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILTTGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVEKLIKQCLKENPQERPTSAQVFDILNSAELVCLTRRILLPKNVIVECMVATHHNSRNASIWLGCGHTDRGQLSFLDLNTEGYTSEEVADSRILCLALVHLPVEKESWIVSGTQSGTLLVINTEDGKKRHTLEKMTDSVTCLYCNSFSKQSKQKNFLLVGTADGKLAIFEDKTVKLKGAAPLKILNIGNVSTPLMCLSESTNSTERNVMWGGCGTKIFSFSNDFTIQKLIETRTSQLFSYAAFSDSNIITVVVDTALYIAKQNSPVVEVWDKKTEKLCGLIDCVHFLREVMVKENKESKHKMSYSGRVKTLCLQKNTALWIGTGGGHILLLDLSTRRLIRVIYNFCNSVRVMMTAQLGSLKNVMLVLGYNRKNTEGTQKQKEIQSCLTVWDINLPHEVQNLEKHIEVRKELAEKMRRTSVE
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Predicted Molecular Mass
210 kDa
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Molecular Weight
Approximately 210 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 70%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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