LSD1 Protein, Human (sf9, His-GST)
Based on 1 publication(s) in Google Scholar
The LSD1 protein is a histone demethylase that modifies histone H3 by demethylating “Lys-4” (H3K4me) and “Lys-9” (H3K9me).It acts as a coactivator or corepressor, oxidizing substrates via FAD to generate imines that are subsequently hydrolyzed.LSD1 Protein, Human (sf9, His-GST) is the recombinant human-derived LSD1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The LSD1 protein is a histone demethylase that modifies histone H3 by demethylating “Lys-4” (H3K4me) and “Lys-9” (H3K9me).It acts as a coactivator or corepressor, oxidizing substrates via FAD to generate imines that are subsequently hydrolyzed.LSD1 Protein, Human (sf9, His-GST) is the recombinant human-derived LSD1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
Background
LSD1, a histone demethylase, exhibits the unique capability to demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, functioning as a versatile coactivator or corepressor depending on the cellular context. Acting through FAD-mediated oxidation and subsequent imine hydrolysis, LSD1 can demethylate mono- and di-methylated H3K4me, thus contributing to the regulation of epigenetic transcriptional activation. In the absence of RCOR1/CoREST, LSD1 alone is unable to demethylate H3K4me on nucleosomes. Additionally, LSD1 serves as a coactivator for androgen receptor (AR)-dependent transcription, participating in the demethylation of H3K9me, a marker associated with epigenetic transcriptional repression. Moreover, LSD1 plays a pivotal role in suppressing neuronal genes and orchestrates the repression of E-cadherin/CDH1, CDN7, and KRT8 through its involvement in the RCOR/GFI/KDM1A/HDAC complex. Beyond histone demethylation, LSD1's diverse interactions extend to demethylating p53/TP53, stabilizing DNMT1, and modifying SOX2. The intricate regulatory role of LSD1 spans embryogenesis, gastrulation, and multilineage blood cell development, emphasizing its significance in shaping the epigenetic landscape and influencing various cellular processes.
Verified Bioactivity
LSD1 demethylates H3K4 to produce formaldehyde, and then in the presence of ammonia, formaldehyde reacts with acetyl linear aniline to produce fluorescent products. The specific activity is 36.565 ng/min/mg.
Publications (1)
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Journal Impact Factor
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Most Recent
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-GST;N-10*His
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Accession
O60341-1 (S172-M852)
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Molecular Construction
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N-term
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10*His-GST
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LSD1 (S172-M852)
Accession # O60341-1 -
C-term
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Protein Length
Partial
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Synonyms
KDM1A; [Histone H3]-Dimethyl-L-Lysine(4) FAD-Dependent Demethylase 1A; Prev. AOF2; FAD-Binding Protein BRAF35-HDAC Complex, 110 KDa Subunit; Prev. KDM1; Lysine-Specific Histone Demethylase 1; Lysine-Specific Histone Demethylase 1A; Lysine-Specific Histone
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AA Sequence
SGVEGAAFQSRLPHDRMTSQEAACFPDIISGPQQTQKVFLFIRNRTLQLWLDNPKIQLTFEATLQQLEAPYNSDTVLVHRVHSYLERHGLINFGIYKRIKPLPTKKTGKVIIIGSGVSGLAAARQLQSFGMDVTLLEARDRVGGRVATFRKGNYVADLGAMVVTGLGGNPMAVVSKQVNMELAKIKQKCPLYEANGQAVPKEKDEMVEQEFNRLLEATSYLSHQLDFNVLNNKPVSLGQALEVVIQLQEKHVKDEQIEHWKKIVKTQEELKELLNKMVNLKEKIKELHQQYKEASEVKPPRDITAEFLVKSKHRDLTALCKEYDELAETQGKLEEKLQELEANPPSDVYLSSRDRQILDWHFANLEFANATPLSTLSLKHWDQDDDFEFTGSHLTVRNGYSCVPVALAEGLDIKLNTAVRQVRYTASGCEVIAVNTRSTSQTFIYKCDAVLCTLPLGVLKQQPPAVQFVPPLPEWKTSAVQRMGFGNLNKVVLCFDRVFWDPSVNLFGHVGSTTASRGELFLFWNLYKAPILLALVAGEAAGIMENISDDVIVGRCLAILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGSSGNDYDLMAQPITPGPSIPGAPQPIPRLFFAGEHTIRNYPATVHGALLSGLREAGRIADQFLGAMYTLPRQATPGVPAQQSPSM
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Predicted Molecular Mass
103.3 kDa
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Molecular Weight
Approximately 93 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
1.Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 2 mM DTT, pH 8.0, 10% Glycerol
, 5% trehalose, 5% mannitol and 0.01% Tween 80.
2.Supplied as a 0.22 μm filtered solution of 20 mM Tris, 5000 mM NaCl, 10% Glycerol, 1 mM TCEP, pH 8.0.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (240 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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