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  3. Enzymes & Regulators Kinases
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  5. MAP4K3 Protein, Human (S170A, sf9, GST, Flag)

MAP4K3 Protein, Human (S170A, sf9, GST, Flag)

Cat. No.: HY-P703250
Handling Instructions Technical Support

MAP4K3 Protein, Human (S170A, sf9, GST, Flag) is the recombinant human-derived MAP4K3, expressed by sf9 insect cells , with GST, Flag labeled tag.

For research use only. We do not sell to patients.

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Description

MAP4K3 Protein, Human (S170A, sf9, GST, Flag) is the recombinant human-derived MAP4K3, expressed by sf9 insect cells , with GST, Flag labeled tag.
Background

MAP4K3 is a serine/threonine kinase that plays a role in the response to environmental stress. It appears to act upstream of the JUN N-terminal pathway (by similar: PubMed:9275185). As an activator of the Hippo signaling pathway, which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis, MAP4K3 acts in parallel to and is partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, establishing MAP4Ks as components of the expanded Hippo pathway (by similar: PubMed:26437443).
Species

Human
Source

Sf9 insect cells
Tag

GST;Flag
Accession

Q8IVH8-1 (M1-G380, S170A)
Gene ID

8491
Protein Length

Partial
Synonyms
Mitogen-activated protein kinase kinase kinase kinase 3; Germinal center kinase-related protein kinase; GLK; MAPK/ERK kinase kinase kinase 3; MEK kinase kinase 3; MEKKK 3; MAP4K3; RAB8IPL1; Homo sapiens; Human; GLK; Kinase; Serine/threonine-protein kinase; Transferase; 2.7.11.1
AA Sequence

MNPGFDLSRRNPQEDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQEIIMMKDCKHPNIVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQIAYVSRETLQGLYYLHSKGKMHRDIKGANILLTDNGHVKLADFGVSAQITATIAKRKAFIGTPYWMAPEVAAVERKGGYNQLCDLWAVGITAIELAELQPPMFDLHPMRALFLMTKSNFQPPKLKDKMKWSNSFHHFVKMALTKNPKKRPTAEKLLQHPFVTQHLTRSLAIELLDKVNNPDHSTYHDFDDDDPEPLVAVPHRIHSTSRNVREEKTRSEITFGQVKFDPPLRKETEPHHELPDSDGFLDSSEEIYYTARSNLDLQLEYG

Predicted Molecular Mass
71 kDa
Purity

≥ 80%, as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

MAP4K3 Protein, Human (S170A, sf9, GST, Flag) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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