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MBL1 Protein, Rat (HEK293, Fc)

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Based on 1 Customer Validation

MBL1 protein is a calcium-dependent lectin that participates in innate immunity by binding to mannose, fucose and N-acetylglucosamine on microorganisms and activating the lectin complement pathway.MBL1 Protein, Rat (HEK293, Fc) is the recombinant rat-derived MBL1 protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for MBL1 Protein, Rat (HEK293, Fc)
  • Experimental Validation Results for MBL1 Protein, Rat (HEK293, Fc)
  • MBL1 Protein, Rat (HEK293, Fc) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

MBL1 protein is a calcium-dependent lectin that participates in innate immunity by binding to mannose, fucose and N-acetylglucosamine on microorganisms and activating the lectin complement pathway.MBL1 Protein, Rat (HEK293, Fc) is the recombinant rat-derived MBL1 protein, expressed by HEK293 , with C-hFc labeled tag.

Background

MBL1, a calcium-dependent lectin, plays a crucial role in the innate immune response by binding to mannose, fucose, and N-acetylglucosamine moieties on various microorganisms, thereby activating the lectin complement pathway. Beyond its involvement in host defense, MBL1 exhibits affinity for late apoptotic cells, apoptotic blebs, and necrotic cells, facilitating their uptake by macrophages. The protein forms homotrimers and assembles into higher oligomeric complexes through the association of two, three, or more homotrimers, a process occurring in the endoplasmic reticulum. This oligomerization pattern underscores the structural complexity of MBL1. Additionally, MBL1 interacts with MASP1 and MASP2, further connecting its role in lectin-mediated immune responses and complement activation.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Mannan at 2μg/mL (100 μL/well) can bind Rat MBL1. The ED50 for this effect is 1.308 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Mannan at 2 μg/mL (100 μL/well) can bind Rat MBL1.The ED50 for this effect is 1.308 μg/mL.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag C-hFc
  • Accession

    P19999 (S18-A238)

  • Gene ID
    24548  [NCBI]
  • Molecular Construction
    • N-term
    • MBL1 (S18-A238)
      Accession # P19999
    • hFc
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    Mannose-binding protein A; Mannan-binding protein; MBP-A; Ra-reactive factor polysaccharide-binding component p28B; RaRF p28B; Mbl1

  • AA Sequence

    SGSQTCEETLKTCSVIACGRDGRDGPKGEKGEPGQGLRGLQGPPGKLGPPGSVGAPGSQGPKGQKGDRGDSRAIEVKLANMEAEINTLKSKLELTNKLHAFSMGKKSGKKFFVTNHERMPFSKVKALCSELRGTVAIPRNAEENKAIQEVAKTSAFLGITDEVTEGQFMYVTGGRLTYSNWKKDEPNDHGSGEDCVTIVDNGLWNDISCQASHTAVCEFPA

  • Molecular Weight

    Approximately 56 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Mannan at 2 μg/mL (100 μL/well) can bind Rat MBL1.The ED50 for this effect is 1.308 μg/mL.

  • MBL1 Protein, Rat (HEK293, Fc) Product Image
    1/16
  • Experimental Validation Results for MBL1 Protein, Rat (HEK293, Fc)
  • Experimental Validation Results for MBL1 Protein, Rat (HEK293, Fc)
  • MBL1 Protein, Rat (HEK293, Fc) Product Image