MBL1 Protein, Rat (HEK293, Fc)

MBL1 protein is a calcium-dependent lectin that participates in innate immunity by binding to mannose, fucose and N-acetylglucosamine on microorganisms and activating the lectin complement pathway. MBL1 Protein, Rat (HEK293, Fc) is the recombinant rat-derived MBL1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of MBL1 Protein, Rat (HEK293, Fc) is 221 a.a., with molecular weight of ~56-60 kDa.

MBL1, a calcium-dependent lectin, plays a crucial role in the innate immune response by binding to mannose, fucose, and N-acetylglucosamine moieties on various microorganisms, thereby activating the lectin complement pathway. Beyond its involvement in host defense, MBL1 exhibits affinity for late apoptotic cells, apoptotic blebs, and necrotic cells, facilitating their uptake by macrophages. The protein forms homotrimers and assembles into higher oligomeric complexes through the association of two, three, or more homotrimers, a process occurring in the endoplasmic reticulum. This oligomerization pattern underscores the structural complexity of MBL1. Additionally, MBL1 interacts with MASP1 and MASP2, further connecting its role in lectin-mediated immune responses and complement activation.

Measured by its binding ability in a functional ELISA. Immobilized Mannan at 2μg/mL (100 μL/well) can bind Rat MBL1. The ED₅₀ for this effect is 1.308 μg/mL.

Rat

HEK293

C-hFc

P19999 (S18-A238)

24548  [NCBI]

SGSQTCEETLKTCSVIACGRDGRDGPKGEKGEPGQGLRGLQGPPGKLGPPGSVGAPGSQGPKGQKGDRGDSRAIEVKLANMEAEINTLKSKLELTNKLHAFSMGKKSGKKFFVTNHERMPFSKVKALCSELRGTVAIPRNAEENKAIQEVAKTSAFLGITDEVTEGQFMYVTGGRLTYSNWKKDEPNDHGSGEDCVTIVDNGLWNDISCQASHTAVCEFPA

Approximately 56-60 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.