MBP/MalE Protein, E.coli (Myc, His)

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The MBP/MalE protein is an important element in the ABC transporter complex MalEFGK and is dedicated to the active import of maltose and maltodextrin. This multifunctional protein displays binding affinity for maltose and higher maltodextrins such as maltotriose. MBP/MalE Protein, E.coli (Myc, His) is the recombinant E. coli-derived MBP/MalE protein, expressed by E. coli , with C-Myc, N-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: E.coli
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

The MBP/MalE protein is an important element in the ABC transporter complex MalEFGK and is dedicated to the active import of maltose and maltodextrin. This multifunctional protein displays binding affinity for maltose and higher maltodextrins such as maltotriose. MBP/MalE Protein, E.coli (Myc, His) is the recombinant E. coli-derived MBP/MalE protein, expressed by E. coli , with C-Myc, N-10*His labeled tag.

Background

MBP/MalE protein functions as a crucial component of the ABC transporter complex MalEFGK, specializing in the active import of maltose and maltodextrins. This versatile protein exhibits a binding affinity for maltose as well as higher maltodextrins, including maltotriose. As part of this transporter complex, MBP/MalE plays a pivotal role in the efficient and selective uptake of maltose and related substrates, contributing to the cellular processes associated with maltose metabolism.

Technical Parameters

  • Species E.coli
  • Source E. coli
  • Tag C-Myc;N-10*His
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • 10*His
    • MBP (K27-K396)
      Accession # P0AEX9
    • Myc
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    MBP; Myelin A1 Protein; Myelin Basic Protein; Golli-MBP; Myelin Membrane Encephalitogenic Protein

  • AA Sequence

    KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTRITK

  • Predicted Molecular Mass

    48.2 kDa

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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