MDO Protein, Mycobacterium sp. (His)
MDO (methanol dehydrogenase) is responsible for oxidizing methanol to produce formaldehyde. Although the in vivo electron acceptor remains unidentified, in vitro experiments indicate that N,N-dimethyl-4-nitrosaniline (NDMA) can serve as a suitable electron acceptor and is subsequently reduced to 4-(hydroxyamino) -N,N-Dimethylaniline. MDO Protein, Mycobacterium sp. (His) is the recombinant MDO protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Others
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
MDO (methanol dehydrogenase) is responsible for oxidizing methanol to produce formaldehyde. Although the in vivo electron acceptor remains unidentified, in vitro experiments indicate that N,N-dimethyl-4-nitrosaniline (NDMA) can serve as a suitable electron acceptor and is subsequently reduced to 4-(hydroxyamino) -N,N-Dimethylaniline. MDO Protein, Mycobacterium sp. (His) is the recombinant MDO protein, expressed by E. coli , with N-6*His labeled tag.
Methanol dehydrogenase (MDO) is an essential enzyme that catalyzes the oxidation of methanol to produce formaldehyde. While the specific in vivo electron acceptor remains unknown, in vitro studies have demonstrated the ability of N,N-dimethyl-4-nitrosoaniline (NDMA) to serve this function, being reduced to 4-(hydroxylamino)-N,N-dimethylaniline in the process. Remarkably, MDO exhibits versatility in substrate utilization, showcasing comparable activity with ethanol and formaldehyde as it does with methanol. Additionally, the enzyme displays weak activity with methylamine as a substrate. This broad substrate specificity suggests MDO's potential role in diverse metabolic pathways and highlights its adaptability in accommodating different alcohol and amine substrates, emphasizing its significance in cellular metabolism and the oxidation of various organic compounds.
Technical Parameters
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Species Others
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Source E. coli
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Tag N-6*His
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Accession
C5MRT8 (M1-Y423)
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Gene ID/
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Molecular Construction
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N-term
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6*His
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MDO (M1-Y423)
Accession # C5MRT8 -
C-term
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Protein Length
Full Length
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Synonyms
Methanol:N,N-dimethyl-4-nitrosoaniline oxidoreductase; EC:1.1.99.37; MDO; Methanol dehydrogenase (nicotinoprotein); Methanol:NDMA oxidoreductase
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AA Sequence
MAIELNQIWDFPIKEFHPFPRALLGVGAHDIIGVEAKNLGFKRTLLMTTGLRGSGIIEELTGKIEYQGVEVVLYDKVESNPKDYNVMEAAALYQQERCDSIISIGGGSSHDAAKGARVVIAHDGRNINEFEGFAKSTNKQNPPHIAVSTTAGTGSETSWAYVITDTSDMEHPHKWVGFDEATIVTLAIDDPLLYYTCPQHFTAYCGFDVLAHGSEPYVSRLDFAPSLGNALYSVELVAKHLREAVFEPRNLKAREGMMNAQYIAGQAFNSGGLGIVHSISHAVSAFFDSHHGLNNAIALPRVWEYNLPSRYERYAQLATAMGVDTRNMTTVQAADAAVEAAIRLSQDVGIPDNFSQVRVDSYDKNRMNTGKYAGKGEVIKGDDKSVLAISEHIQGDWCTPGNPREVTVDSMIPVVGHAINGTY
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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