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  3. Transferases (EC 2)
  4. metK Protein, Shigella sonnei (His)

metK Protein, Shigella sonnei (His)

Cat. No.: HY-P701983
COA Handling Instructions

The metK protein plays a crucial role in cellular processes by catalyzing the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. This enzymatic activity involves a two-step process, first synthesizing AdoMet and then hydrolyzing tripolyphosphate. metK Protein, Shigella sonnei (His) is the recombinant metK protein, expressed by E. coli , with N-6*His labeled tag. The total length of metK Protein, Shigella sonnei (His) is 384 a.a., .

For research use only. We do not sell to patients.

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Size Price Stock Quantity
10 μg $240 In-stock
50 μg $620 In-stock
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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

The metK protein plays a crucial role in cellular processes by catalyzing the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. This enzymatic activity involves a two-step process, first synthesizing AdoMet and then hydrolyzing tripolyphosphate. metK Protein, Shigella sonnei (His) is the recombinant metK protein, expressed by E. coli , with N-6*His labeled tag. The total length of metK Protein, Shigella sonnei (His) is 384 a.a., .

Background

MetK, or methionine adenosyltransferase, plays a crucial role in cellular metabolism by catalyzing the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. This enzymatic reaction involves two sequential steps: the initial synthesis of AdoMet and the subsequent hydrolysis of tripolyphosphate, occurring before the release of AdoMet from the enzyme. S-adenosylmethionine serves as a primary methyl donor in various biochemical reactions, participating in processes such as DNA methylation, protein methylation, and the biosynthesis of polyamines and certain secondary metabolites. MetK's activity is pivotal for maintaining cellular methylation potential and ensuring the availability of AdoMet, a key cofactor involved in numerous essential metabolic pathways. It has to succinctly outline MetK's role in the synthesis of S-adenosylmethionine, emphasizing its significance in cellular methylation processes and broader metabolic regulation.

Species

Others

Source

E. coli

Tag

N-6*His

Accession

Q3YXS9 (M1-K384)

Gene ID

75205223

Molecular Construction
N-term
6*His
metK (M1-K384)
Accession # Q3YXS9
C-term
Synonyms
metK; S-adenosylmethionine synthase; AdoMet synthase; MAT; Methionine adenosyltransferase
Molecular Weight

Approximately 44 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

metK Protein, Shigella sonnei (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
metK Protein, Shigella sonnei (His)
Cat. No.:
HY-P701983
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