MMP-13 Protein, Human (HEK293)

The MMP-13 protein plays a role in the degradation of extracellular matrix proteins, especially fibrillar collagen, fibronectin, TNC, and ACAN. It cleaves triple-helical collagen, preferentially cleaves type II collagen, and can also target other collagen types. MMP-13 Protein, Human (HEK293) is the recombinant human-derived MMP-13 protein, expressed by HEK293 , with tag free.

MMP-13 (Matrix Metalloproteinase 13) emerges as a pivotal player in extracellular matrix modulation, wielding its influence over various proteins such as fibrillar collagen, fibronectin, TNC, and ACAN. It exhibits notable proficiency in cleaving triple helical collagens, particularly type I, type II, and type III, with the highest activity observed with soluble type II collagen. MMP-13's multifaceted role extends beyond matrix degradation; it may also act on key regulatory proteins, including TGFB1 and CCN2, thereby influencing processes such as wound healing, tissue remodeling, cartilage degradation, and bone development. Its indispensability in embryonic bone development and ossification underscores its significance, while its involvement in fracture healing through endochondral ossification highlights its dynamic contributions to physiological processes. Moreover, MMP-13 plays a role in keratinocyte migration during wound healing and is implicated in cell migration and tumor invasion, reflecting its diverse impact on cellular functions.

Human

HEK293

Tag Free

P45452 (M1-C471)

4322

Full Length

MHPGVLAAFLFLSWTHCRALPLPSGGDEDDLSEEDLQFAERYLRSYYHPTNLAGILKENAASSMTERLREMQSFFGLEVTGKLDDNTLDVMKKPRCGVPDVGEYNVFPRTLKWSKMNLTYRIVNYTPDMTHSEVEKAFKKAFKVWSDVTPLNFTRLHDGIADIMISFGIKEHGDFYPFDGPSGLLAHAFPPGPNYGGDAHFDDDETWTSSSKGYNLFLVAAHEFGHSLGLDHSKDPGALMFPIYTYTGKSHFMLPDDDVQGIQSLYGPGDEDPNPKHPKTPDKCDPSLSLDAITSLRGETMIFKDRFFWRLHPQQVDAELFLTKSFWPELPNRIDAAYEHPSHDLIFIFRGRKFWALNGYDILEGYPKKISELGLPKEVKKISAAVHFEDTGKTLLFSGNQVWRYDDTNHIMDKDYPRLIEEDFPGIGDKVDAVYEKNGYIYFFNGPIQFEYSIWSNRIVRVMPANSILWC

Approximately 44.2 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 1 mM DTT, 200 mM NaCl, 20% glycerol.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.