MMP-2 Protein, Human (HEK293)

1. Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 and the specific activity is > 1,000 pmoles/min/µg. (Activation description: The proenzyme needs to be activated by APMA for an activated form). 2. Measured by its binding ability in a functional ELISA. Immobilized Human MMP-2 at 2 μg/ml (100 μl/well) can bind Human TIMP2 hFc and the EC₅₀ is 6.0-30.0 ng/mL.

Human

HEK293

Tag Free

NP_004521.1 (A30-C660)

4313  [NCBI]

Approximately 72 kDa

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 0.05% Brij-35, 150 mM NaCl, 5 mM CaCl₂, 50 mM Tris, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.