Mca-PLGL-Dpa-AR-NH2
Based on 1 Customer Validation
Mca-PLGL-Dpa-AR-NH2 (Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2) is a synthetic fluorescent substrate mainly used for in vitro enzymatic activity assays of MMP-7 (detection at 393 nm after excitation at 328 nm). Mca-PLGL-Dpa-AR-NH2 is specifically cleaved and hydrolyzed by MMP-7 at the Gly-Leu peptide bond, allowing enzymatic activity to be monitored via fluorometry or HPLC (DMSO is usually added to ensure sufficient dissolution when determining kinetic parameters). Mca-PLGL-Dpa-AR-NH2 is used to accurately evaluate the catalytic activity and pH dependence of recombinant mature human MMP-7 in vitro. Mca-PLGL-Dpa-AR-NH2 is suitable for research on the mechanisms of tumor metastasis and invasion, particularly in fields such as prostate cancer, colon cancer, lung cancer and breast cancer.
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- Pureté : 99.65%
- CAS No.: 140430-53-1
- Formule: C49H68N14O15
- Masse moléculaire:1093.15
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Stockage:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
IC50 & Target
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MMP-7 |
In Vitro
Mca-PLGL-Dpa-AR-NH2 (1.5 μM; 25°C) is a fluorescent substrate of MMP-7, and its hydrolysis is detectable by changes in fluorescence intensity at 393 nm after excitation at 328 nm[1].
Mca-PLGL-Dpa-AR-NH2 (1.5 mM) acts as a fluorogenic substrate for recombinant human MMP-7 and can be used to determine the catalytic activity of this enzyme. Its maximal intrinsic kcat/Kmo is 1.20 × 105 M-1s-1, with the peak occurring at pH 5-8[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
393
Chemical Information
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CAS No. 140430-53-1
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Appearance Solid
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Masse moléculaire 1093.15
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Formule C49H68N14O15
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Color Light yellow to yellow
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SMILES
N=C(N)NCCC[C@@H](C(N)=O)NC([C@H](C)NC([C@H](CNC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O)NC([C@H](CC(C)C)NC(CNC([C@H](CC(C)C)NC([C@H]2N(C(CC(C3=CC=C(OC)C=C3O4)=CC4=O)=O)CCC2)=O)=O)=O)=O)=O)=O
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Synonyms
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2
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Sequence
{Mca}-Pro-Leu-Gly-Leu-{Dpa}-Ala-Arg-NH2
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Sequence Shortening
{Mca}-PLGL-{Dpa}-AR-NH2
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (91.48 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Pureté et documentation
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Fiche technique (292 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Oneda H, et al. Inhibitory effects of green tea catechins on the activity of human matrix metalloproteinase 7 (matrilysin). J Biochem. 2003;133(5):571-576. [Content Brief]
[2]. Muta Y, et al. Expression in Escherichia coli, refolding, and purification of the recombinant mature form of human matrix metalloproteinase 7 (MMP-7). Biosci Biotechnol Biochem. 2010;74(12):2515-2517. [Content Brief]
[3]. Muta Y, et al. Inhibitory effects of lignans on the activity of human matrix metalloproteinase 7 (matrilysin). J Agric Food Chem. 2004;52(19):5888-5894. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 0.9148 mL | 4.5739 mL | 9.1479 mL | 22.8697 mL |
| 5 mM | 0.1830 mL | 0.9148 mL | 1.8296 mL | 4.5739 mL | |
| 10 mM | 0.0915 mL | 0.4574 mL | 0.9148 mL | 2.2870 mL | |
| 15 mM | 0.0610 mL | 0.3049 mL | 0.6099 mL | 1.5246 mL | |
| 20 mM | 0.0457 mL | 0.2287 mL | 0.4574 mL | 1.1435 mL | |
| 25 mM | 0.0366 mL | 0.1830 mL | 0.3659 mL | 0.9148 mL | |
| 30 mM | 0.0305 mL | 0.1525 mL | 0.3049 mL | 0.7623 mL | |
| 40 mM | 0.0229 mL | 0.1143 mL | 0.2287 mL | 0.5717 mL | |
| 50 mM | 0.0183 mL | 0.0915 mL | 0.1830 mL | 0.4574 mL | |
| 60 mM | 0.0152 mL | 0.0762 mL | 0.1525 mL | 0.3812 mL | |
| 80 mM | 0.0114 mL | 0.0572 mL | 0.1143 mL | 0.2859 mL |
Mca-PLGL-Dpa-AR-NH2 Related Classifications
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