MOCAc-PLGL(Dpa)AR  (Synonyms: Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2)

MOCAc-PLGL (Dpa) AR (Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH₂) is a synthetic fluorescent substrate mainly used for in vitro enzymatic activity assays of MMP-7 (detection at 393 nm after excitation at 328 nm). MOCAc-PLGL (Dpa) AR is specifically cleaved and hydrolyzed by MMP-7 at the Gly-Leu peptide bond, allowing enzymatic activity to be monitored via fluorometry or HPLC (DMSO is usually added to ensure sufficient dissolution when determining kinetic parameters). MOCAc-PLGL (Dpa) AR is used to accurately evaluate the catalytic activity and pH dependence of recombinant mature human MMP-7 in vitro. MOCAc-PLGL (Dpa) AR is suitable for research on the mechanisms of tumor metastasis and invasion, particularly in fields such as prostate cancer, colon cancer, lung cancer and breast cancer^[1][2][3].\n

MOCAc-PLGL(Dpa)AR (1.5 μM; 25°C) is a fluorescent substrate of MMP-7, and its hydrolysis is detectable by changes in fluorescence intensity at 393 nm after excitation at 328 nm^[1].
MOCAc-PLGL(Dpa)AR (1.5 mM) acts as a fluorogenic substrate for recombinant human MMP-7 and can be used to determine the catalytic activity of this enzyme. Its maximal intrinsic k_cat/K_mo is 1.20 × 10⁵ M^-1s^-1, with the peak occurring at pH 5-8^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1093.15

C₄₉H₆₈N₁₄O₁₅

140430-53-1

Solid

Light yellow to yellow

393

328

N=C(N)NCCC[C@@H](C(N)=O)NC([C@H](C)NC([C@H](CNC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O)NC([C@H](CC(C)C)NC(CNC([C@H](CC(C)C)NC([C@H]2N(C(CC(C3=CC=C(OC)C=C3O4)=CC4=O)=O)CCC2)=O)=O)=O)=O)=O)=O

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Oneda H, et al. Inhibitory effects of green tea catechins on the activity of human matrix metalloproteinase 7 (matrilysin). J Biochem. 2003;133(5):571-576.  [Content Brief]

  [2]. Muta Y, et al. Expression in Escherichia coli, refolding, and purification of the recombinant mature form of human matrix metalloproteinase 7 (MMP-7). Biosci Biotechnol Biochem. 2010;74(12):2515-2517.

  [3]. Muta Y, et al. Inhibitory effects of lignans on the activity of human matrix metalloproteinase 7 (matrilysin). J Agric Food Chem. 2004;52(19):5888-5894.