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NAALADL1 Protein, Human (HEK293, His)

Customer Review

Based on 1 Customer Validation

NAALADL1 is an aminopeptidase with broad substrate specificity and shows lower activity towards substrates containing Asp or Glu at the P2' position or Pro at the P3' position. It is worth noting that it lacks activity against substrates with Pro at the P3' position and Asp or Glu at the P2' position. NAALADL1 Protein, Human (HEK293, His) is the recombinant human-derived NAALADL1 protein, expressed by HEK293 , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for NAALADL1 Protein, Human (HEK293, His)
  • Experimental Validation Results for NAALADL1 Protein, Human (HEK293, His)
  • NAALADL1 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

NAALADL1 is an aminopeptidase with broad substrate specificity and shows lower activity towards substrates containing Asp or Glu at the P2' position or Pro at the P3' position. It is worth noting that it lacks activity against substrates with Pro at the P3' position and Asp or Glu at the P2' position. NAALADL1 Protein, Human (HEK293, His) is the recombinant human-derived NAALADL1 protein, expressed by HEK293 , with N-His labeled tag.

Background

NAALADL1 is an aminopeptidase exhibiting a broad substrate specificity, although it demonstrates lower activity with substrates containing Asp or Glu in the P2' position, or Pro in the P3' position. Notably, it lacks activity with substrates featuring both Pro in the P3' position and Asp or Glu in the P2' position. In addition, NAALADL1 does not possess carboxypeptidase activity and is devoid of dipeptidyl-peptidase IV type activity. These enzymatic characteristics emphasize the nuanced substrate preferences and limitations of NAALADL1, shedding light on its role as an aminopeptidase with specific substrate interactions and catalytic properties.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human NAALADL1, at 2 μg/mL (100μL/well) can bind Anti-NAALADL1 antibody. The ED50 for this effect is 0.1597 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Human NAALADL1, at 2 μg/mL (100μL/well) can bind Anti-NAALADL1 antibody. The ED50 for this effect is 0.1597 μg/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag N-His
  • Accession

    Q9UQQ1-1 (P29-L740)

  • Gene ID
    10004  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • NAALADL1 (P29-L740)
      Accession # Q9UQQ1
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    NAALADL1; Ileal Dipeptidylpeptidase; N-Acetylated Alpha-Linked Acidic Dipeptidase Like 1; Ileal Peptidase I100; N-Acetylated-Alpha-Linked Acidic Dipeptidase-Like Protein; NAALADase L; Aminopeptidase NAALADL1; NAALADASEL; HILAP; I100; 100 KDa Ileum Brush B

  • AA Sequence

    PKKANSLAPQDLDLEILETVMGQLDAHRIRENLRELSREPHLASSPRDEDLVQLLLQRWKDPESGLDSAEASTYEVLLSFPSQEQPNVVDIVGPTGGIIHSCHRTEENVTGEQGGPDVVQPYAAYAPSGTPQGLLVYANRGAEEDFKELQTQGIKLEGTIALTRYGGVGRGAKAVNAAKHGVAGVLVYTDPADINDGLSSPDETFPNSWYLPPSGVERGSYYEYFGDPLTPYLPAVPSSFRVDLANVSGFPPIPTQPIGFQDARDLLCNLNGTLAPATWQGALGCHYRLGPGFRPDGDFPADSQVNVSVYNRLELRNSSNVLGIIRGAVEPDRYVLYGNHRDSWVHGAVDPSSGTAVLLELSRVLGTLLKKGTWRPRRSIVFASWGAEEFGLIGSTEFTEEFFNKLQERTVAYINVDISVFANATLRVQGTPPVQSVVFSATKEIRSPGPGDLSIYDNWIRYFNRSSPVYGLVPSLGSLGAGSDYAPFVHFLGISSMDIAYTYDRSKTSARIYPTYHTAFDTFDYVDKFLDPGFSSHQAVARTAGSVILRLSDSFFLPLKVSDYSETLRSFLQAAQQDLGALLEQHSISLGPLVTAVEKFEAEAAALGQRISTLQKGSPDPLQVRMLNDQLMLLERTFLNPRAFPEERYYSHVLWAPRTGSVVTFPGLSNACSRARDTASGSEAWAEVQRQLSIVVTALEGAAATLRPVADL

  • Molecular Weight

    Approximately 90 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Human NAALADL1, at 2 μg/mL (100μL/well) can bind Anti-NAALADL1 antibody. The ED50 for this effect is 0.1597 μg/mL.

  • NAALADL1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for NAALADL1 Protein, Human (HEK293, His)
  • Experimental Validation Results for NAALADL1 Protein, Human (HEK293, His)
  • NAALADL1 Protein, Human (HEK293, His) Product Image