NAALADL1 Protein, Human (HEK293, His)

NAALADL1 is an aminopeptidase with broad substrate specificity and shows lower activity towards substrates containing Asp or Glu at the P2' position or Pro at the P3' position. It is worth noting that it lacks activity against substrates with Pro at the P3' position and Asp or Glu at the P2' position. NAALADL1 Protein, Human (HEK293, His) is the recombinant human-derived NAALADL1 protein, expressed by HEK293 , with N-His labeled tag.

NAALADL1 is an aminopeptidase exhibiting a broad substrate specificity, although it demonstrates lower activity with substrates containing Asp or Glu in the P2' position, or Pro in the P3' position. Notably, it lacks activity with substrates featuring both Pro in the P3' position and Asp or Glu in the P2' position. In addition, NAALADL1 does not possess carboxypeptidase activity and is devoid of dipeptidyl-peptidase IV type activity. These enzymatic characteristics emphasize the nuanced substrate preferences and limitations of NAALADL1, shedding light on its role as an aminopeptidase with specific substrate interactions and catalytic properties.

Measured by its binding ability in a functional ELISA. Immobilized Human NAALADL1, at 2 μg/mL (100μL/well) can bind Anti-NAALADL1 antibody. The ED₅₀ for this effect is 0.1597 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Human NAALADL1, at 2 μg/mL (100μL/well) can bind Anti-NAALADL1 antibody. The ED₅₀ for this effect is 0.1597 μg/mL.

Human

HEK293

N-His

Q9UQQ1-1 (P29-L740)

10004  [NCBI]

Extracellular Domain

PKKANSLAPQDLDLEILETVMGQLDAHRIRENLRELSREPHLASSPRDEDLVQLLLQRWKDPESGLDSAEASTYEVLLSFPSQEQPNVVDIVGPTGGIIHSCHRTEENVTGEQGGPDVVQPYAAYAPSGTPQGLLVYANRGAEEDFKELQTQGIKLEGTIALTRYGGVGRGAKAVNAAKHGVAGVLVYTDPADINDGLSSPDETFPNSWYLPPSGVERGSYYEYFGDPLTPYLPAVPSSFRVDLANVSGFPPIPTQPIGFQDARDLLCNLNGTLAPATWQGALGCHYRLGPGFRPDGDFPADSQVNVSVYNRLELRNSSNVLGIIRGAVEPDRYVLYGNHRDSWVHGAVDPSSGTAVLLELSRVLGTLLKKGTWRPRRSIVFASWGAEEFGLIGSTEFTEEFFNKLQERTVAYINVDISVFANATLRVQGTPPVQSVVFSATKEIRSPGPGDLSIYDNWIRYFNRSSPVYGLVPSLGSLGAGSDYAPFVHFLGISSMDIAYTYDRSKTSARIYPTYHTAFDTFDYVDKFLDPGFSSHQAVARTAGSVILRLSDSFFLPLKVSDYSETLRSFLQAAQQDLGALLEQHSISLGPLVTAVEKFEAEAAALGQRISTLQKGSPDPLQVRMLNDQLMLLERTFLNPRAFPEERYYSHVLWAPRTGSVVTFPGLSNACSRARDTASGSEAWAEVQRQLSIVVTALEGAAATLRPVADL

Approximately 95 kDa due to the glycosylation

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.