Nectin-4 Protein, Human (HEK293, His, MALS verified)
Based on 1 Customer Validation
The Nectin-4 Protein IgV domain is pivotal in cell adhesion, participating in trans-homophilic and -heterophilic interactions, notably with NECTIN1. While it doesn't serve as an alpha-herpesvirus entry receptor, it functions as a receptor for measles virus, enabling virus entry into host cells. This dual role underscores the importance of Nectin-4 in cell adhesion and its specific role as a measles virus infection receptor. Nectin-4 Protein, Human (HEK293, His, MALS verified) is the recombinant human-derived Nectin-4 protein, expressed by HEK293, with C-10*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The Nectin-4 Protein IgV domain is pivotal in cell adhesion, participating in trans-homophilic and -heterophilic interactions, notably with NECTIN1. While it doesn't serve as an alpha-herpesvirus entry receptor, it functions as a receptor for measles virus, enabling virus entry into host cells. This dual role underscores the importance of Nectin-4 in cell adhesion and its specific role as a measles virus infection receptor. Nectin-4 Protein, Human (HEK293, His, MALS verified) is the recombinant human-derived Nectin-4 protein, expressed by HEK293, with C-10*His labeled tag.
Background
The Nectin-4 Protein IgV domain plays a crucial role in cell adhesion, engaging in both trans-homophilic and -heterophilic interactions. Specifically, it forms interactions with NECTIN1, contributing to cellular adhesion processes. However, it does not function as a receptor for alpha-herpesvirus entry into cells. In the context of microbial infection, the Nectin-4 IgV domain acts as a receptor for measles virus, facilitating the entry of the virus into host cells. This highlights the dual role of Nectin-4 in cell adhesion and its significance as a specific receptor for measles virus infection.
Verified Bioactivity
1.Immobilized Human Nectin-4 at 1 μg/mL (100 μL/well) can bind Enfortumab (HY-P99016). The ED50 for this effect is 0.046 ng/mL.
2.Immobilized Human Nectin-4 at 5 μg/mL (100 μL/well) can bind Human Nectin-1 (HY-P704809). The ED50 for this effect is 2.107 μg/mL.
3.Nectin-4 Antibody (HY-P86995) captured on CM5 chip via anti-mouse antibodies surface can bind Human Nectin-4 with an affinity constant of 4.49 nM as determined in a SPR assay.(Biacore 8K).
4.Loaded Human Nectin-4 on HIS1K Biosensor, can bind Human Nectin-1 (HY-P704809) with an affinity constant of 59.8 nM as determined in BLI assay.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-10*His
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Accession
Q96NY8-1 (G32-V351)
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Gene ID81607
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Molecular Construction
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N-term
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Nectin-4 (G32-V351)
Accession # Q96NY8-1 -
10*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
PVRL4; Nectin-4; Ig superfamily receptor LNIR; Poliovirus receptor-related protein 4; PRR4; LNIR; Nectin4
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AA Sequence
GELETSDVVTVVLGQDAKLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEGRVEQPPPPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTTSSRSFKHSRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDQRITHILHVSFLAEASVRGLEDQNLWHIGREGAMLKCLSEGQPPPSYNWTRLDGPLPSGVRVDGDTLGFPPLTTEHSGIYVCHVSNEFSSRDSQVTVDVLDPQEDSGKQVDLVSASVV
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Predicted Molecular Mass
35.5 kDa
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Molecular Weight
Approximately 40-45 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation. Approximately 30-46 kDa, verified by SEC-MALS.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.; ≥ 90%, as determined by MALS.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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