NEIL1 Protein, Human (His)
Based on 1 Customer Validation
NEIL1 is a critical base excision repair enzyme that recognizes and eliminates damaged bases and preferentially selects oxidized pyrimidines such as thymine glycol, Fapy, and 5-hydroxyuracil. Although NEIL1 has limited activity toward 8-oxoguanine, it has excellent AP lyase activity and introduces nicking by eliminating β-δ. NEIL1 Protein, Human (His) is the recombinant human-derived NEIL1 protein, expressed by E. coli , with C-His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
NEIL1 is a critical base excision repair enzyme that recognizes and eliminates damaged bases and preferentially selects oxidized pyrimidines such as thymine glycol, Fapy, and 5-hydroxyuracil. Although NEIL1 has limited activity toward 8-oxoguanine, it has excellent AP lyase activity and introduces nicking by eliminating β-δ. NEIL1 Protein, Human (His) is the recombinant human-derived NEIL1 protein, expressed by E. coli , with C-His labeled tag.
Background
NEIL1, a crucial participant in base excision repair, plays a pivotal role in addressing DNA damage induced by oxidation or exposure to mutagenic agents. Functioning as a DNA glycosylase, NEIL1 recognizes and eliminates damaged bases, exhibiting a preference for oxidized pyrimidines such as thymine glycol, formamidopyrimidine (Fapy), and 5-hydroxyuracil. Although it shows marginal activity towards 8-oxoguanine, NEIL1 excels in AP (apurinic/apyrimidinic) lyase activity, introducing nicks in the DNA strand. Through beta-delta elimination, it cleaves the DNA backbone, generating a single-strand break at the site of the removed base, encompassing both 3'- and 5'-phosphates. Notably, NEIL1 demonstrates DNA glycosylase/lyase activity towards mismatched uracil and thymine, particularly in U:C and T:C mismatches. Moreover, its specific binding to 5-hydroxymethylcytosine (5hmC) suggests that NEIL1 serves as a specialized reader of this modified DNA base.
Verified Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human NEIL1 at 2 μg/mL (100 μL/well) can bind Anti- NEIL1 antibody, The ED50 for this effect is 25.76 ng/mL.
Technical Parameters
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Species Human
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Source E. coli
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Tag C-His
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Accession
Q96FI4 (M1-S390)
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Molecular Construction
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N-term
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NEIL1 (M1-S390)
Accession # Q96FI4 -
His
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C-term
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Protein Length
Full Length
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Synonyms
NEIL1; Endonuclease 8-Like 1; Nei Like DNA Glycosylase 1; Nei-Like Protein 1; FPG1; Nei Homolog 1; HFPG1; FLJ22402; NEI1; NEH1; DNA-(Apurinic Or Apyrimidinic Site) Lyase Neil1; Nei Endonuclease VIII-Like 1 (E. Coli); DNA Endonuclease Eight-Like Glycosylas
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AA Sequence
MPEGPELHLASQFVNEACRALVFGGCVEKSSVSRNPEVPFESSAYRISASARGKELRLILSPLPGAQPQQEPLALVFRFGMSGSFQLVPREELPRHAHLRFYTAPPGPRLALCFVDIRRFGRWDLGGKWQPGRGPCVLQEYQQFRENVLRNLADKAFDRPICEALLDQRFFNGIGNYLRAEILYRLKIPPFEKARSVLEALQQHRPSPELTLSQKIRTKLQNPDLLELCHSVPKEVVQLGGKGYGSESGEEDFAAFRAWLRCYGMPGMSSLQDRHGRTIWFQGDPGPLAPKGRKSRKKKSKATQLSPEDRVEDALPPSKAPSRTRRAKRDLPKRTATQRPEGTSLQQDPEAPTVPKKGRRKGRQAASGHCRPRKVKADIPSLEPEGTSAS
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Molecular Weight
Approximately 45 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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