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  5. Neuropilin-1 Protein, Human (HEK293, His)

Neuropilin-1 Protein, Human (HEK293, His)

Cat. No.: HY-P70147
COA Handling Instructions

Neuropilin-1 protein plays an important role by binding to VEGF-165, possibly inhibiting its cellular binding and inducing apoptosis. This dual function suggests its role in regulating VEGF-mediated processes. Neuropilin-1 Protein, Human (HEK293, His) is the recombinant human-derived Neuropilin-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Neuropilin-1 Protein, Human (HEK293, His) is 623 a.a., with molecular weight of 85-100 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Neuropilin-1 protein plays an important role by binding to VEGF-165, possibly inhibiting its cellular binding and inducing apoptosis. This dual function suggests its role in regulating VEGF-mediated processes. Neuropilin-1 Protein, Human (HEK293, His) is the recombinant human-derived Neuropilin-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Neuropilin-1 Protein, Human (HEK293, His) is 623 a.a., with molecular weight of 85-100 kDa.

Background

The Neuropilin-1 protein assumes a significant role as it binds to VEGF-165, potentially inhibiting its binding to cells and inducing apoptosis by sequestering VEGF-165. This dual functionality suggests Neuropilin-1's involvement in the regulation of VEGF-mediated cellular processes. Additionally, Neuropilin-1 may interact with various members of the semaphorin family, indicating its versatility in binding to different ligands. Notably, its expression appears to exert an adverse effect on blood vessel number and integrity, suggesting a potential role in angiogenesis and vascular development. The diverse interactions and regulatory effects of Neuropilin-1 underscore its importance in orchestrating complex cellular processes and its potential implications in angiogenesis and apoptotic pathways.

Biological Activity

1.Measured by its binding ability in a functional ELISA. Immobilized human Neuropilin-1, at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF165 protein. The ED50 for this effect is 14.96 ng/mL.
2.Immobilized Recombinant Human / Cynomolgus VEGF / VEGFA / VEGF165 Protein at 2 μg/mL (100 μL/well) can bind Neuropilin-1, Human with a linear range of 32-160 μg/mL.
3.Measured by its ability to inhibit the cell growth of MCF-7 human breast cancer cell line. The ED50 for this effect is 13.44 ng/mL, corresponding to a specific activity is 7.44×104 U/mg.
4.Measured in a cell proliferation assay using HUVEC cells. The ED50 for this effect is 154.3 ng/mL, corresponding to a specific activity is 6.48×103 units/mg.

  • Measured by its binding ability in a functional ELISA. Immobilized human Neuropilin-1, at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF165 protein. The ED50 for this effect is 14.96 ng/mL.
Species

Human

Source

HEK293

Tag

C-His

Accession

O14786-2 (F22-K644)

Gene ID
Molecular Construction
N-term
Neuropilin-1 (F22-K644)
Accession # O14786-2
His
C-term
Synonyms
rHuNeuropilin-1, His ; Neuropilin-1, CD304; NRP1; NRPNP1; VEGF165R; BDCA4
AA Sequence

FRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKYPNSLECTYIVFAPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSVSEDFKCMEALGMESGEIHSDQITASSQYSTNWSAERSRLNYPENGWTPGEDSYREWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYKIDVSSNGEDWITIKEGNKPVLFQGNTNPTDVVVAVFPKPLITRFVRIKPATWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITSSNQGDRNWMPENIRLVTSRSGWALPPAPHSYINEWLQIDLGEEKIVRGIIIQGGKHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSGIK

Molecular Weight

85-100 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Neuropilin-1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Neuropilin-1 Protein, Human (HEK293, His)
Cat. No.:
HY-P70147
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