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Neuropilin-1 Protein, Human (HEK293, His)

3 Cited Publications
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Based on 3 publication(s) in Google Scholar

Neuropilin-1 protein plays an important role by binding to VEGF-165, possibly inhibiting its cellular binding and inducing apoptosis. This dual function suggests its role in regulating VEGF-mediated processes. Neuropilin-1 Protein, Human (HEK293, His) is the recombinant human-derived Neuropilin-1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Neuropilin-1 Protein, Human (HEK293, His)
  • Experimental Validation Results for Neuropilin-1 Protein, Human (HEK293, His)
  • Neuropilin-1 Protein, Human (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Top Publications Citing

Use of MCE Products

Biological Activity

Description

Neuropilin-1 protein plays an important role by binding to VEGF-165, possibly inhibiting its cellular binding and inducing apoptosis. This dual function suggests its role in regulating VEGF-mediated processes. Neuropilin-1 Protein, Human (HEK293, His) is the recombinant human-derived Neuropilin-1 protein, expressed by HEK293 , with C-His labeled tag.

Background

The Neuropilin-1 protein assumes a significant role as it binds to VEGF-165, potentially inhibiting its binding to cells and inducing apoptosis by sequestering VEGF-165. This dual functionality suggests Neuropilin-1's involvement in the regulation of VEGF-mediated cellular processes. Additionally, Neuropilin-1 may interact with various members of the semaphorin family, indicating its versatility in binding to different ligands. Notably, its expression appears to exert an adverse effect on blood vessel number and integrity, suggesting a potential role in angiogenesis and vascular development. The diverse interactions and regulatory effects of Neuropilin-1 underscore its importance in orchestrating complex cellular processes and its potential implications in angiogenesis and apoptotic pathways.

Verified Bioactivity

1.Measured by its binding ability in a functional ELISA. Immobilized human Neuropilin-1, at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF165 protein. The ED50 for this effect is 14.96 ng/mL.
2.Immobilized Recombinant Human / Cynomolgus VEGF / VEGFA / VEGF165 Protein at 2 μg/mL (100 μL/well) can bind Neuropilin-1, Human with a linear range of 32-160 μg/mL.
3.Measured by its ability to inhibit the cell growth of MCF-7 human breast cancer cell line. The ED50 for this effect is 13.44 ng/mL, corresponding to a specific activity is 7.44×104 U/mg.
4.Measured in a cell proliferation assay using HUVEC cells. The ED50 for this effect is 154.3 ng/mL, corresponding to a specific activity is 6.48×103 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized human Neuropilin-1, at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF165 protein. The ED50 for this effect is 14.96 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    AAH07533.1 (F22-K644)

  • Gene ID
    8829  [NCBI]
  • Molecular Construction
    • N-term
    • Neuropilin-1 (F22-K644)
      Accession # AAH07533.1
    • His
    • C-term
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    NRP1; Transmembrane Receptor; Neuropilin 1; CD304 Antigen; VEGF165R; DKFZp686M1967; NRP; Neuropilin; Neuropilin-1; BDCA4; CD304; NP1; Vascular Endothelial Cell Growth Factor 165 Receptor

  • AA Sequence

    FRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKYPNSLECTYIVFAPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSVSEDFKCMEALGMESGEIHSDQITASSQYSTNWSAERSRLNYPENGWTPGEDSYREWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYKIDVSSNGEDWITIKEGNKPVLFQGNTNPTDVVVAVFPKPLITRFVRIKPATWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITSSNQGDRNWMPENIRLVTSRSGWALPPAPHSYINEWLQIDLGEEKIVRGIIIQGGKHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSGIK

  • Molecular Weight

    Approximately 75-116 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized human Neuropilin-1, at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF165 protein. The ED50 for this effect is 14.96 ng/mL.

  • Neuropilin-1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Neuropilin-1 Protein, Human (HEK293, His)
  • Experimental Validation Results for Neuropilin-1 Protein, Human (HEK293, His)
  • Neuropilin-1 Protein, Human (HEK293, His) Product Image