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Neuropilin-1 Protein, Rat (HEK293, His)

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Based on 1 Customer Validation

Neuropilin-1 protein is a cell surface receptor that plays multiple roles in cardiovascular development, angiogenesis, and neuronal circuit formation. It mediates chemical repulsion by recognizing ligands with CendR motifs, leading to internalization and vascular leakage. Neuropilin-1 Protein, Rat (HEK293, His) is the recombinant rat-derived Neuropilin-1 protein, expressed by HEK293, with C-6*His labeled tag and K811R, P812-G828 delet.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Neuropilin-1 Protein, Rat (HEK293, His)
  • Experimental Validation Results for Neuropilin-1 Protein, Rat (HEK293, His)
  • Neuropilin-1 Protein, Rat (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Neuropilin-1 protein is a cell surface receptor that plays multiple roles in cardiovascular development, angiogenesis, and neuronal circuit formation. It mediates chemical repulsion by recognizing ligands with CendR motifs, leading to internalization and vascular leakage. Neuropilin-1 Protein, Rat (HEK293, His) is the recombinant rat-derived Neuropilin-1 protein, expressed by HEK293, with C-6*His labeled tag and K811R, P812-G828 delet.

Background

Neuropilin-1 Protein is a cell-surface receptor that plays diverse roles in the development of the cardiovascular system, angiogenesis, neuronal circuit formation, and organogenesis. It acts as a mediator for the chemorepulsant activity of semaphorins and recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands, leading to cellular internalization and vascular leakage. It binds to various ligands including semaphorin 3A, PLGF-2 isoform of PGF, VEGF165 isoform of VEGFA, and VEGFB. When coexpressed with KDR, it enhances the binding of VEGF165 to KDR and promotes chemotaxis. Neuropilin-1 regulates VEGF-induced angiogenesis and plays a crucial role in motor neuron axon guidance and cell body migration, particularly during the caudal migration of facial motor neurons in embryonic development. It is also involved in the regulation of mitochondrial iron transport through its interaction with ABCB8/MITOSUR. Neuropilin-1 forms homodimers and heterodimers with NRP2, interacts with PLXNB1, FER, VEGFA, and ABCB8/MITOSUR in mitochondria.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Rat Neuropilin-1 at 2 µg/mL can bind Human VEGF165. The ED50 for this effect is 0.8699 nM.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Rat Neuropilin-1 at 2 µg/mL can bind Human VEGF165. The ED50 for this effect is 0.8699 nM

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q9QWJ9 (F22-D854, K811R, ΔP812-G828)

  • Gene ID
    246331
  • Protein Length

    Partial

  • Synonyms

    NRP1; Transmembrane Receptor; Neuropilin 1; CD304 Antigen; VEGF165R; DKFZp686M1967; NRP; Neuropilin; Neuropilin-1; BDCA4; CD304; NP1; Vascular Endothelial Cell Growth Factor 165 Receptor

  • AA Sequence

    FRSDKCGGTIKIENPGYLTSPGYPHSYHPSEKCEWLIQAPEPYQRIMINFNPHFDLEDRDCKYDYVEVIDGENEGGRLWGKFCGKIAPSPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTAPTGVIKSPGFPEKYPNSLECTYIIFAPKMSEIILEFESFDLEQDSNPPGGVFCRYDRLEIWDGFPEVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSISEDFKCMEALGMESGEIHSDQITASSQYGTNWSVERSRLNYPENGWTPGEDSYREWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYRVDISSNGEDWITLKEGNKAIIFQGNTNPTDVVFGVFPKPLITRFVRIKPASWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITASNQGDRNWMPENIRLVTSRTGWALPPSPHPYINEWLQVDLGDEKIVRGVIIQGGKHRENKVFMRKFKIAYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRAFTPLSTRFIRIYPERATHSGLGLRMELLGCEVEVPTAGPTTPNGNPVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTIIDSTIQSEFPTYGFNCEFGWGSHKTFCHWEHDSHAQLRWRVLTSKTGPIQDHTGDGNFIYSQADENQKGKVARLVSPVVYSQSSAHCMTFWYHMSGSHVGTLRVKLHYQKPEEYDQLVWMVVGHQGDHWKEGRVLLHKSLKLYQVIFEGEIGKGNLGGIAVDDISINNHIPQEDCARSTPGYEEGKGDKNISRKPGNVLKTLD

  • Predicted Molecular Mass

    91.8 kDa

  • Molecular Weight

    Approximately 98-130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<0.1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Rat Neuropilin-1 at 2 µg/mL can bind Human VEGF165. The ED50 for this effect is 0.8699 nM

  • Neuropilin-1 Protein, Rat (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Neuropilin-1 Protein, Rat (HEK293, His)
  • Experimental Validation Results for Neuropilin-1 Protein, Rat (HEK293, His)
  • Neuropilin-1 Protein, Rat (HEK293, His) Product Image