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Neurotrophin-3 Protein, Human (HEK293)

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Based on 1 Customer Validation

Neurotrophin-3 (NT-3) is crucial for promoting the survival of sensory neurons, specifically those linked to visceral and proprioceptive functions. Its role highlights NT-3's significance in maintaining and supporting the functionality of these specialized sensory cells, emphasizing its potential as a key regulator in the complex network governing essential physiological sensory processes. Neurotrophin-3 Protein, Human (HEK293) is the recombinant human-derived Neurotrophin-3 protein, expressed by HEK293 , with tag free.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Neurotrophin-3 Protein, Human (HEK293)
  • Experimental Validation Results for Neurotrophin-3 Protein, Human (HEK293)
  • Neurotrophin-3 Protein, Human (HEK293) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Neurotrophin-3 (NT-3) is crucial for promoting the survival of sensory neurons, specifically those linked to visceral and proprioceptive functions. Its role highlights NT-3's significance in maintaining and supporting the functionality of these specialized sensory cells, emphasizing its potential as a key regulator in the complex network governing essential physiological sensory processes. Neurotrophin-3 Protein, Human (HEK293) is the recombinant human-derived Neurotrophin-3 protein, expressed by HEK293 , with tag free.

Background

Neurotrophin-3 (NT-3) emerges as a crucial factor in fostering the survival of sensory neurons, particularly those associated with visceral and proprioceptive functions. Its role in supporting the viability of these specialized sensory cells underscores the significance of NT-3 in orchestrating the intricate mechanisms that contribute to the maintenance and functionality of visceral and proprioceptive neuronal populations. The specific actions of NT-3 in promoting neuronal survival shed light on its potential as a key regulator in the complex network governing sensory processes essential for proper physiological functioning.

Verified Bioactivity

1. Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with TrkB. The ED50 for this effect is < 40 ng/mL.
2. Human TrkC immobilized on CM5 Chip can bind Human NT-3 with an affinity constant of ≤0.06 nM as determined in a SPR assay.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with TrkB. The ED50 for this effect is 36.23 ng/mL, corresponding to a specific activity is 2.760×104 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag Tag Free
  • Accession

    P20783-1 (Y139-T257)

  • Gene ID
    4908
  • Molecular Construction
    • N-term
    • Neurotrophin-3 (Y139-T257)
      Accession # P20783-1
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    NTF3; Neurotrophin-3; Neurotrophin 3; NGF-2; NGF2; HDNF; Nerve Growth Factor 2; NT-3; Neurotrophic Factor; NT3

  • AA Sequence

    YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT

  • Predicted Molecular Mass

    13.6 kDa

  • Molecular Weight

    Approximately 13-14 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Structure/Form

    Monomer

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<0.1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with TrkB. The ED50 for this effect is 36.23 ng/mL, corresponding to a specific activity is 2.760×104 units/mg.

  • Neurotrophin-3 Protein, Human (HEK293) Product Image
    1/16
  • Experimental Validation Results for Neurotrophin-3 Protein, Human (HEK293)
  • Experimental Validation Results for Neurotrophin-3 Protein, Human (HEK293)
  • Neurotrophin-3 Protein, Human (HEK293) Product Image