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Neurotrophin-4 Protein, Human (HEK293)

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Based on 1 Customer Validation

Neurotrophin-4 (NT-4) protein serves as a vital target-derived survival factor for peripheral sensory sympathetic neurons, emphasizing its crucial role in promoting their survival and maintenance. The specific and targeted actions of NT-4 underscore its significance as a neurotrophic factor in the intricate mechanisms governing the well-being of peripheral neurons within the sympathetic nervous system. Neurotrophin-4 Protein, Human (HEK293) is the recombinant human-derived Neurotrophin-4 protein, expressed by HEK293 , with tag free.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Neurotrophin-4 Protein, Human (HEK293)
  • Experimental Validation Results for Neurotrophin-4 Protein, Human (HEK293)
  • Neurotrophin-4 Protein, Human (HEK293) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

Neurotrophin-4 (NT-4) protein serves as a vital target-derived survival factor for peripheral sensory sympathetic neurons, emphasizing its crucial role in promoting their survival and maintenance. The specific and targeted actions of NT-4 underscore its significance as a neurotrophic factor in the intricate mechanisms governing the well-being of peripheral neurons within the sympathetic nervous system. Neurotrophin-4 Protein, Human (HEK293) is the recombinant human-derived Neurotrophin-4 protein, expressed by HEK293 , with tag free.

Background

Neurotrophin-4 (NT-4) protein operates as a crucial target-derived survival factor specifically for peripheral sensory sympathetic neurons. Its pivotal role lies in supporting the survival and maintenance of these neurons, emphasizing NT-4's significance in promoting the health and functionality of peripheral sensory sympathetic neurons. The specific and targeted action of NT-4 underscores its role as a neurotrophic factor, playing a key part in the intricate mechanisms that govern the survival and well-being of peripheral neurons within the sympathetic nervous system.

Verified Bioactivity

Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with TrkB. The ED50 for this effect is 2.337 ng/mL, corresponding to a specific activity is 4.279×105 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with TrkB. The ED50 for this effect is 2.337 ng/mL, corresponding to a specific activity is 4.279×105 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag Tag Free
  • Accession

    P34130 (G81-A210)

  • Gene ID
    4909
  • Molecular Construction
    • N-term
    • Neurotrophin-4 (G81-A210)
      Accession # P34130
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    NTF4; Neutrophic Factor 4; Prev. NTF5; NT-4; Neurotrophin-4; NT-5; NT-4/5; Neurotrophic Factor 5; GLC1O; GLC10; Neurotrophin 5 (Neurotrophin 4/5); NT4; Neurotrophic Factor 4; NT5; Neurotrophin 4; Neurotrophin-5

  • AA Sequence

    GVSETAPASRRGELAVCDAVSGWVTDRRTAVDLRGREVEVLGEVPAAGGSPLRQYFFETRCKADNAEEGGPGAGGGGCRGVDRRHWVSECKAKQSYVRALTADAQGRVGWRWIRIDTACVCTLLSRTGRA

  • Predicted Molecular Mass

    13.9 kDa

  • Molecular Weight

    Approximately 14 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 0.1% TFA, 20% Acetonitrile, pH 2.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Mass (in vial) Mass (in vial)
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Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with TrkB. The ED50 for this effect is 2.337 ng/mL, corresponding to a specific activity is 4.279×105 units/mg.

  • Neurotrophin-4 Protein, Human (HEK293) Product Image
    1/16
  • Experimental Validation Results for Neurotrophin-4 Protein, Human (HEK293)
  • Experimental Validation Results for Neurotrophin-4 Protein, Human (HEK293)
  • Neurotrophin-4 Protein, Human (HEK293) Product Image