1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. NIT2 Protein, Arabidopsis thaliana (His)

NIT2 Protein, Arabidopsis thaliana (His)

Cat. No.: HY-P701989
Handling Instructions

The NIT2 protein has the extraordinary ability to catalyze the conversion of 3-indole-acetonitrile into 3-indole-acetic acid, an important plant hormone involved in many physiological processes. This transition is significant because it represents a key step in the biosynthesis of indole-3-acetic acid, a key regulator of plant growth and development. NIT2 Protein, Arabidopsis thaliana (His) is the recombinant NIT2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of NIT2 Protein, Arabidopsis thaliana (His) is 339 a.a., .

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  • Biological Activity

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Description

The NIT2 protein has the extraordinary ability to catalyze the conversion of 3-indole-acetonitrile into 3-indole-acetic acid, an important plant hormone involved in many physiological processes. This transition is significant because it represents a key step in the biosynthesis of indole-3-acetic acid, a key regulator of plant growth and development. NIT2 Protein, Arabidopsis thaliana (His) is the recombinant NIT2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of NIT2 Protein, Arabidopsis thaliana (His) is 339 a.a., .

Background

NIT2 protein possesses the remarkable ability to catalyze the conversion of indole-3-acetonitrile into indole-3-acetic acid, which is a vital plant hormone involved in numerous physiological processes. This transformation is significant as it represents a key step in the biosynthesis of indole-3-acetic acid, a crucial regulator of plant growth and development. NIT2's enzymatic activity contributes to the homeostasis of indole-3-acetic acid levels, which in turn influences plant organogenesis, cell elongation, root development, and responses to environmental stimuli. Understanding the role and regulation of NIT2 protein in this metabolic pathway can provide insights into plant hormone signaling and potentially be harnessed for agricultural applications, such as enhancing crop yield or stress tolerance.

Species

Others

Source

E. coli

Tag

N-6*His

Accession

P32962 (M1-K339)

Gene ID

823555

Molecular Construction
N-term
6*His
NIT2 (M1-K339)
Accession # P32962
C-term
Synonyms
NIT2; Nitrilase 2
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

NIT2 Protein, Arabidopsis thaliana (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
NIT2 Protein, Arabidopsis thaliana (His)
Cat. No.:
HY-P701989
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