nitA Protein, Rhodococcus rhodochrous (His)
nitA protein enzymatically acts on aliphatic nitriles, specifically acrylonitrile, crotononitrile, and glutaronitrile substrates. nitA Protein, Rhodococcus rhodochrous (His) is the recombinant nitA, expressed by E. coli, with His labeled tag.
- Species: Others
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
nitA protein enzymatically acts on aliphatic nitriles, specifically acrylonitrile, crotononitrile, and glutaronitrile substrates. nitA Protein, Rhodococcus rhodochrous (His) is the recombinant nitA, expressed by E. coli, with His labeled tag.
Background
nitA protein demonstrates its enzymatic activity on aliphatic nitriles, specifically targeting substrates like acrylonitrile, crotononitrile, and glutaronitrile.
Technical Parameters
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Species Others
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Source E. coli
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Tag His
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Accession
Q1W2L4 (M1-K369)
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Gene ID/
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Protein Length
Partial
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Synonyms
Nitrilase; EC:3.5.5.1; nitA
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AA Sequence
MVSYNSKFLAATVQAEPVWLDADATIDKSIGIIEEAAQKGASLIAFPEVFIPGYPYWAWLGDVKYSLSFTSRYHENSLELGDDRMRRLQLAARRNKIALVMGYSEREAGSRYLSQVFIDERGEIVANRRKLKPTHVERTIYGEGNGTDFLTHDFAFGRVGGLNCWEHFQPLSKFMMYSLGEQVHVASWPAMSPLQPDVFQLSIEANATVTRSYAIEGQTFVLCSTQVIGPSAIETFCLNDEQRALLPQGCGWARIYGPDGSELAKPLAEDAEGILYAEIDLEQILLAKAGADPVGHYSRPDVLSVQFDPRNHTPVHRIGIDGRLDVNTRSRVENFRLRQAAEQERQASKRLGTKLFEQSLLAEEPVPAK
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Predicted Molecular Mass
43.4 kDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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