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  3. CD314/NKG2D T Cell CD Proteins NK Cell CD Proteins
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  5. NKG2D/CD314 Protein, Human (Biotinylated, HEK293, His-Avi)

NKG2D/CD314 Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P78183
COA Handling Instructions

The NKG2D/CD314 protein serves as a key activating and costimulatory receptor in immune surveillance, binding to multiple stress-inducing ligands on tumor and virus-infected cells. It plays a dual role by stimulating NK cells and enhancing T cell activation in CD8(+) T cell-mediated adaptive responses. NKG2D/CD314 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived NKG2D/CD314 protein, expressed by HEK293 , with N-His, N-Avi labeled tag. The total length of NKG2D/CD314 Protein, Human (Biotinylated, HEK293, His-Avi) is 139 a.a., with molecular weight of ~19.0 kDa.

For research use only. We do not sell to patients.

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20 μg $245 In-stock
50 μg $540 In-stock
100 μg $918 Get quote
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  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

The NKG2D/CD314 protein serves as a key activating and costimulatory receptor in immune surveillance, binding to multiple stress-inducing ligands on tumor and virus-infected cells. It plays a dual role by stimulating NK cells and enhancing T cell activation in CD8(+) T cell-mediated adaptive responses. NKG2D/CD314 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived NKG2D/CD314 protein, expressed by HEK293 , with N-His, N-Avi labeled tag. The total length of NKG2D/CD314 Protein, Human (Biotinylated, HEK293, His-Avi) is 139 a.a., with molecular weight of ~19.0 kDa.

Background

NKG2D/CD314 protein operates as an activating and costimulatory receptor essential for immunosurveillance, binding to diverse cellular stress-inducible ligands presented on autologous tumor cells and virus-infected cells. It plays a dual role in innate immune responses, stimulating both activating killer (NK) cells and acting as a costimulatory receptor for T-cell receptors (TCR) in CD8(+) T-cell-mediated adaptive immune responses, enhancing T-cell activation. The receptor facilitates perforin-mediated elimination of ligand-expressing tumor cells, and its signaling cascades involve calcium influx, ultimately leading to TNF-alpha expression. Additionally, NKG2D/CD314 participates in NK cell-mediated bone marrow graft rejection and may regulate the differentiation and survival of NK cells. Its ligand-binding capacity extends to various subfamilies of MHC class I-related glycoproteins, including MICA, MICB, RAET1E, RAET1G, RAET1L/ULBP6, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3), and ULBP4. The protein forms homodimers through disulfide linkage and heterohexamers with HCST/DAP10 subunits, a crucial interaction for NK cell surface expression and cytotoxicity induction. Furthermore, it can establish disulfide-bonded heterodimers with CD94 and interacts with CEACAM1, recruiting PTPN6 for VAV1 dephosphorylation, while not interacting with TYROBP.

Biological Activity

Measured by its binding ability in a functional ELISA. When immobilized Biotinylated Human NKG2D His at 1 μg/mL (100μL/Well), Anti-NKG2D Antibody hFc binds with an EC50 of 4.4 ng/mL.

Species

Human

Source

HEK293

Tag

N-His;N-Avi

Accession

P26718 (F78-V216)

Gene ID

100528032  [NCBI]

Molecular Construction
N-term
His-Avi
NKG2D (F78-V216)
Accession # P26718
C-term
Synonyms
CD314; D12S2489E; KLR; NKG2-D; NKG2D
Molecular Weight

Approximately 19.0 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

NKG2D/CD314 Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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NKG2D/CD314 Protein, Human (Biotinylated, HEK293, His-Avi)
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HY-P78183
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