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Non-structural protein 1/NS1 Protein, H1N1 (His)

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Nonstructural protein 1/NS1 plays multiple roles in viral infection.It inhibits cellular pre-mRNA processing by binding to CPSF4 and PABPN1, disrupting normal 3' end processing and halting host protein synthesis.Non-structural protein 1/NS1 Protein, H1N1 (His) is the recombinant Virus-derived Non-structural protein 1/NS1 protein, expressed by E.coli , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Virus
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Non-structural protein 1/NS1 Protein, H1N1 (His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

Nonstructural protein 1/NS1 plays multiple roles in viral infection.It inhibits cellular pre-mRNA processing by binding to CPSF4 and PABPN1, disrupting normal 3' end processing and halting host protein synthesis.Non-structural protein 1/NS1 Protein, H1N1 (His) is the recombinant Virus-derived Non-structural protein 1/NS1 protein, expressed by E.coli , with N-His labeled tag.

Background

The Non-structural protein 1/NS1 serves as a multifaceted regulator during viral infection. It exerts control over post-transcriptional processing of cellular pre-mRNA by binding and inhibiting essential cellular proteins, specifically the 30 kDa cleavage and polyadenylation specificity factor/CPSF4 and the poly(A)-binding protein 2/PABPN1. This interference prevents the normal 3'-end processing of cellular pre-mRNAs, leading to the accumulation of unprocessed pre-mRNAs in the host nucleus, effectively shutting down cellular protein synthesis. NS1 also plays a crucial role in evading the cellular antiviral response by inhibiting TRIM25-mediated RIGI ubiquitination, disrupting the activation of type I interferon genes. Additionally, NS1 interferes with the integrated stress response (ISR) by blocking dsRNA binding by EIF2AK2/PKR, inhibiting stress granule formation, and facilitating viral replication. This multifunctional protein further interacts with various cellular components, including TRIM25, EIF2AK2/PKR, CPSF4, IVNS1ABP, and PABPN1, highlighting its intricate involvement in modulating host cell processes to favor viral replication.

Technical Parameters

  • Species Virus
  • Source E. coli
  • Tag N-His
  • Accession

    C8XP22 (D2-V230)

  • Gene ID
    /
  • Molecular Construction
    • N-term
    • His
    • H1N1 NS1 (D2-V230)
      Accession # C8XP22
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    PTPN11; Protein Tyrosine Phosphatase Non-Receptor Type 11 Isoform 1; Prev. NS1; Tyrosine-Protein Phosphatase Non-Receptor Type; SH-PTP2; Protein-Tyrosine-Phosphatase; PTP2C; Noonan Syndrome 1; BPTP3; CDNA, FLJ92431; SHP-2; PTPN11 Protein; SHP2; METCDS; SH

  • AA Sequence

    DPNTVSSFQVDCFLWHVRKRVADQELGDAPFLDRLRRDQKSLRGRGSTLGLDIETATRAGKQIVERILKEESDEALKMTMASVPASRYLTDMTLEEMSRDWSMLIPKQKVAGPLCIRMDQAIMDKNIILKANFSVIFDRLETLILLRAFTEEGAIVGEISPLPSLPGHTAEDVKNAVGVLIGGLEWNDNTVRVSETLQRFAWRSSNENGRPPLTPKQKREMAGTIRSEV

  • Predicted Molecular Mass

    27.2 kDa

  • Molecular Weight

    Approximately 29 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of PBS, 5% glycerol, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
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Biological Activity (ED50) Biological Activity (ED50)
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ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • Non-structural protein 1/NS1 Protein, H1N1 (His) Product Image