NS1 Protein, Dengue virus 3 (HEK293, His)

The NS1 protein plays a key role in viral budding, association with cell membranes, and facilitating the assembly of viral RNA into nucleocapsids to form mature viral particles. During entry, NS1 induces genome penetration into the host cytoplasm. NS1 Protein, Dengue virus 3 (HEK293, His) is the recombinant Virus-derived NS1 protein, expressed by HEK293 , with N-His labeled tag.

The NS1 protein assumes a pivotal role in virus budding by binding to the cell membrane, facilitating the assembly of the viral RNA into a nucleocapsid that forms the core of a mature virus particle. During virus entry, NS1 may induce genome penetration into the host cytoplasm following hemifusion induced by surface proteins. Notably, NS1 exhibits the ability to migrate to the cell nucleus, where it modulates host functions. Additionally, NS1 counteracts the antiviral effects of host EXOC1 by sequestering and degrading EXOC1 through the proteasome degradation pathway. Furthermore, NS1 disrupts RNA silencing by interfering with host Dicer, highlighting its multifaceted role in manipulating both viral and host cellular processes throughout the infection cycle.

Virus

HEK293

N-His

P27915/AAA99437.1 (D774-A1125)

80527852

Partial

DMGCVINWKGKELKCGSGIFVTNEVHTWTEQYKFQADSPKRVATAIAGAWENGVCGIRSTTRMENLLWKQIANELNYILWENDIKLTVVVGDITGVLEQGKRTLTPQPMELKYSWKTWGLAKIVTAETQNSSFIIDGPSTPECPSASRAWNVWEVEDYGFGVFTTNIWLKLREVYTQLCDHRLMSAAVKDERAVHADMGYWIESQKNGSWKLEKASLIEVKTCTWPKSHTLWSNGVLESDMIIPKSLAGPISQHNHRPGYHTQTAGPWHLGKLELDFNYCEGTTVVISENCGTRGPSLRTTTVSGKLIHEWCCRSCTLPPLRYMGEDGCWYGMEIRPINEKEENMVKSLASA

≥ 90%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.