NS5 Protein, Dengue virus 2 (His)
Based on 1 Customer Validation
NS5 Protein is part of the flavivirus RNA replication complex (RC) composed of viral non-structural proteins and host-cell cofactors. NS5 is the largest flavivirus protein, the most conserved, which act as two domains, the RNA-dependent RNA polymerase (RdRp) and RNA methyltransferase enzyme (MTase). NS5 plays a fundamental role in flavivirus replication, viral RNA methylation, RNA polymerization, and host immune system evasion. NS5 Protein, Dengue virus 2 (His) is the recombinant Virus-derived NS5 protein, expressed by E. coli , with N-His labeled tag.
- Species: Virus
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
NS5 Protein is part of the flavivirus RNA replication complex (RC) composed of viral non-structural proteins and host-cell cofactors. NS5 is the largest flavivirus protein, the most conserved, which act as two domains, the RNA-dependent RNA polymerase (RdRp) and RNA methyltransferase enzyme (MTase). NS5 plays a fundamental role in flavivirus replication, viral RNA methylation, RNA polymerization, and host immune system evasion. NS5 Protein, Dengue virus 2 (His) is the recombinant Virus-derived NS5 protein, expressed by E. coli , with N-His labeled tag.
Background
Non-structural protein 5 (NS5) is part of the flavivirus RNA replication complex (RC) composed of viral non-structural proteins and host-cell cofactors. NS5 is the largest flavivirus protein, the most conserved, which act as two domains, the RNA-dependent RNA polymerase (RdRp) and RNA methyltransferase enzyme (MTase). The DENV-NS5 RdRp domain contributes to the viral replication stages, whereas the MTase initiates viral RNA capping and facilitates polyprotein translation. NS5 plays a fundamental role in viral RNA methylation, RNA polymerization, and host immune system evasion. It functions as a scaffold protein with different binding sites for the host STAT2 and ERC1 proteins, but sharing a requirement for UBR4. NS5 also interacts with hostcell proteins as hSTAT2 and contributes to the evasion of the type I interferon (IFN)-mediated innate immune response, which is the first step of host-cell defense against viral infections. NS5 is a potent antagonist of type I IFN signaling of the DENV proteins by suppressing the human JAK-STAT signaling[1][2][3].
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Virus
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Source E. coli
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Tag N-6*His
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Accession
AAC59275.1 (G2492-Q2787)
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Gene ID/
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Molecular Construction
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N-term
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6*His
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NS5 (G2492-Q2787)
Accession # AAC59275.1 -
C-term
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Protein Length
Partial
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Synonyms
Dengue virus DENV-2 (strain New Guinea C) NS5 (methyltransferase domain) / Nonstructural protein 5 Protein; DENV-NS5 Protein; DENV
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AA Sequence
GTGNIGETLGEKWKSRLNALGKSEFQIYKKSGIQEVDRTLAKEGIKRGETDHHAVSRGSAKLRWFVERNMVTPEGKVVDLGCGRGGWSYYCGGLKNVREVKGLTKGGPGHEEPIPMSTYGWNLVRLQSGVDVFFTPPEKCDTLLCDIGESSPNPTVEAGRTLRVLNLVENWLNNNTQFCIKVLNPYMPSVIEKMEALQRKYGGALVRNPLSRNSTHEMYWVSNASGNIVSSVNMISRMLINRFTMRHKKATYEPDVDLGSGTRNIGIESEIPNLDIIGKRIEKIKQEHETSWHYDQ
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Molecular Weight
Approximately 33 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, 200 mM arginine, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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