2. Recombinant Proteins
  3. Viral Proteins
  4. Influenza Viruses Proteins
  5. Influenza Virus Nucleoprotein
  6. Nucleocapsid/NP Protein, HTNV (sf9, His)

Nucleocapsid/NP Protein, HTNV (sf9, His)

Cat. No.: HY-P76965
COA Handling Instructions

Nucleocapsid/NP belongs to the hantavirus nucleocapsid protein family. Nucleocapsid/NP Protein, HTNV (sf9, His) is the recombinant Virus-derived Nucleocapsid/NP protein, expressed by Sf9 insect cells, with C-His labeled tag. The total length of Nucleocapsid/NP Protein, HTNV (sf9, His) is 429 a.a., with molecular weight of ~49.7 KDa.

For research use only. We do not sell to patients.

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Description

Nucleocapsid/NP belongs to the hantavirus nucleocapsid protein family. Nucleocapsid/NP Protein, HTNV (sf9, His) is the recombinant Virus-derived Nucleocapsid/NP protein, expressed by Sf9 insect cells, with C-His labeled tag. The total length of Nucleocapsid/NP Protein, HTNV (sf9, His) is 429 a.a., with molecular weight of ~49.7 KDa.
Background

Nucleocapsid (NP) is a member of the hantavirus nucleocapsid protein family.
Species

Virus
Source

Sf9 insect cells
Tag

C-His
Accession

Q91LN7 (M1-L429)
Gene ID

/
Molecular Construction
N-term
HTNV NP (M1-L429)
Accession # Q91LN7
His
C-term
Synonyms
Hantaan virus HTNV (strain 84FLi) Nucleocapsid / NP Protein (His)
Molecular Weight

Approximately 49.7 kDa.
Purity

Greater than 80% as determined by reducing SDS-PAGE.
Appearance

Solution.
Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 20 % glycerol, pH 8.0.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping

Shipping with dry ice.
Documentation

COA (232 KB)

Handling Instructions (2659 KB)

Nucleocapsid/NP Protein, HTNV (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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