OXCT1 Protein, Human (His)
Based on 1 Customer Validation
OXCT1 Protein, Human (His) is the recombinant human-derived OXCT1, expressed by E. coli , with N-6*His labeled tag. ,
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
OXCT1 Protein, Human (His) is the recombinant human-derived OXCT1, expressed by E. coli , with N-6*His labeled tag. ,
OXCT1 is a key enzyme in ketone body catabolism, catalyzing the first and rate-limiting step of ketone body utilization in extrahepatic tissues. It transfers coenzyme A (CoA) from a donor thiolester (succinyl-CoA) to an acceptor carboxylate (acetoacetate), producing acetoacetyl-CoA. Acetoacetyl-CoA is further metabolized by acetoacetyl-CoA thiolase into two acetyl-CoA molecules, which enter the citric acid cycle for energy production. The enzyme forms a dimer where both subunits can form enzyme-CoA thiolester intermediates, but only one subunit is capable of transferring the CoA moiety to the acceptor carboxylate (3-oxo acid) to generate a new acyl-CoA. The formation of the enzyme-CoA intermediate occurs via an unstable anhydride species between the carboxylate groups of the enzyme and substrate (By similarity).
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
P55809-1 (T40-N520)
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Gene ID5019
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Protein Length
Full Length of Isoform-1 Mature Protein
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Synonyms
OXCT1; SCOT-S; Prev. OXCT; Epididymis Secretory Sperm Binding Protein; SCOT; Succinyl-CoA:3-Ketoacid CoA Transferase; Succinyl-CoA:3-Ketoacid Coenzyme A Transferase 1, Mitochondrial; Succinyl CoA:3-Oxoacid CoA Transferase; Somatic-Type Succinyl-CoA:3-Oxoa
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AA Sequence
TKFYTDPVEAVKDIPDGATVLVGGFGLCGIPENLIDALLKTGVKGLTAVSNNAGVDNFGLGLLLRSKQIKRMVSSYVGENAEFERQYLSGELEVELTPQGTLAERIRAGGAGVPAFYTPTGYGTLVQEGGSPIKYNKDGSVAIASKPREVREFNGQHFILEEAITGDFALVKAWKADRAGNVIFRKSARNFNLPMCKAAETTVVEVEEIVDIGAFAPEDIHIPQIYVHRLIKGEKYEKRIERLSIRKEGDGEAKSAKPGDDVRERIIKRAALEFEDGMYANLGIGIPLLASNFISPNITVHLQSENGVLGLGPYPRQHEADADLINAGKETVTILPGASFFSSDESFAMIRGGHVDLTMLGAMQVSKYGDLANWMIPGKMVKGMGGAMDLVSSAKTKVVVTMEHSAKGNAHKIMEKCTLPLTGKQCVNRIITEKAVFDVDKKKGLTLIELWEGLTVDDVQKSTGCDFAVSPKLMPMQQIAN
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Predicted Molecular Mass
56.1 kDa
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Molecular Weight
Approximately 61 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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