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  4. PAG-1 Protein, Bovine (His)

PAG-1 Protein, Bovine (His)

Cat. No.: HY-P71551
Handling Instructions

PAG-1, a protein of interest, exhibits apparent proteolytic inactivity. PAG-1 Protein, Bovine (His) is the recombinant bovine-derived PAG-1 protein, expressed by E. coli , with N-His labeled tag. The total length of PAG-1 Protein, Bovine (His) is 327 a.a., with molecular weight of ~40.7 kDa.

For research use only. We do not sell to patients.

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Description

PAG-1, a protein of interest, exhibits apparent proteolytic inactivity. PAG-1 Protein, Bovine (His) is the recombinant bovine-derived PAG-1 protein, expressed by E. coli , with N-His labeled tag. The total length of PAG-1 Protein, Bovine (His) is 327 a.a., with molecular weight of ~40.7 kDa.
Background

PAG-1, a protein of interest, is characterized by its apparent proteolytic inactivity.
Species

Bovine
Source

E. coli
Tag

N-His
Accession

Q29432 (54R-380V)
Gene ID

281964  [NCBI]
Molecular Construction
N-term
His
PAG-1 (54R-380V)
Accession # Q29432
C-term
Synonyms
Pregnancy-associated glycoprotein 1; PAG 1; EC 3.4.23.-; Pregnancy-specific protein B; PSP-B
AA Sequence

RGSNLTTHPLRNIKDLVYMGNITIGTPPQEFQVVFDTASSDLWVPSDFCTSPACSTHVRFRHLQSSTFRLTNKTFRITYGSGRMKGVVVHDTVRIGNLVSTDQPFGLSIEEYGFEGRIYDGVLGLNYPNISFSGAIPIFDKLKNQRAISEPVFAFYLSKDEREGSVVMFGGVDHRYYEGELNWVPLIQAGDWSVHMDRISIERKIIACSDGCKALVDTGTSDIVGPRRLVNNIHRLIGAIPRGSEHYVPCSEVNTLPSIVFTINGINYPVPGRAYILKDDRGRCYTTFQENRVSSSTETWYLGDVFLRLYFSVFDRGNDRIGLARAV

Molecular Weight

Approximately 40.7 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

PAG-1 Protein, Bovine (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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