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PAH Protein, Human (D415N, sf9, His)

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PAH protein is a key enzyme involved in amino acid metabolism. Its main function is to catalyze the hydroxylation of L-phenylalanine and convert it into L-tyrosine. PAH Protein, Human (D415N, sf9, His) is the recombinant human-derived PAH protein, expressed by Sf9 insect cells , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

PAH protein is a key enzyme involved in amino acid metabolism. Its main function is to catalyze the hydroxylation of L-phenylalanine and convert it into L-tyrosine. PAH Protein, Human (D415N, sf9, His) is the recombinant human-derived PAH protein, expressed by Sf9 insect cells , with N-His labeled tag.

Background

PAH protein is a key enzyme involved in amino acid metabolism. Its primary function is to catalyze the hydroxylation of L-phenylalanine, converting it into L-tyrosine. This enzymatic activity is a critical step in the conversion of one amino acid to another, specifically contributing to the synthesis of tyrosine from phenylalanine. This process is essential for various biological processes that require the production of tyrosine, which serves as a building block for the synthesis of important molecules like neurotransmitters, thyroid hormones, and melanin. The activity of PAH protein plays a pivotal role in maintaining proper amino acid balance and supporting various physiological functions in the body.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-His
  • Accession

    P00439 (M1-K452, D415N)

  • Gene ID
    5053  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • PAH (M1-K452, D415N)
      Accession # P00439
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    PAH; PH; Phenylalanine Hydroxylase; L-Phenylalanine Hydroxylase; Phenylalanine-4-Hydroxylase; PAH Protein; Phe-4-Monooxygenase; PKU1; Phenylalanine 4-Monooxygenase; PKU

  • AA Sequence

    MSTAVLENPGLGRKLSDFGQETSYIEDNCNQNGAISLIFSLKEEVGALAKVLRLFEENDVNLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILRHDIGATVHELSRDKKKDTVPWFPRTIQELDRFANQILSYGAELDADHPGFKDPVYRARRKQFADIAYNYRHGQPIPRVEYMEEEKKTWGTVFKTLKSLYKTHACYEYNHIFPLLEKYCGFHEDNIPQLEDVSQFLQTCTGFRLRPVAGLLSSRDFLGGLAFRVFHCTQYIRHGSKPMYTPEPDICHELLGHVPLFSDRSFAQFSQEIGLASLGAPDEYIEKLATIYWFTVEFGLCKQGDSIKAYGAGLLSSFGELQYCLSEKPKLLPLELEKTAIQNYTVTEFQPLYYVAESFNDAKEKVRNFAATIPRPFSVRYNPYTQRIEVLDNTQQLKILADSINSEIGILCSALQKIK

  • Predicted Molecular Mass

    52.8 kDa

  • Molecular Weight

    Approximately 50-53 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE or Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 0.5 M NaCl, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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