PNLIP/Pancreatic lipase Protein, Mouse (His-SUMO)
Pancreatic lipase (PNLIP) plays a crucial role in fat metabolism by enzymatically breaking down dietary lipids and preferentially selecting long-chain fatty acid esters at positions 1 and 3. This results in the production of mainly 2-monoacylglycerol and free fatty acids. PNLIP/Pancreatic lipase Protein, Mouse (His-SUMO) is the recombinant mouse-derived PNLIP/Pancreatic lipase protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
- Species: Mouse
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
Pancreatic lipase (PNLIP) plays a crucial role in fat metabolism by enzymatically breaking down dietary lipids and preferentially selecting long-chain fatty acid esters at positions 1 and 3. This results in the production of mainly 2-monoacylglycerol and free fatty acids. PNLIP/Pancreatic lipase Protein, Mouse (His-SUMO) is the recombinant mouse-derived PNLIP/Pancreatic lipase protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
Background
The Pancreatic lipase (PNLIP) protein is integral to fat metabolism, exhibiting a vital role in the enzymatic breakdown of dietary lipids. PNLIP displays a preference for splitting esters of long-chain fatty acids at positions 1 and 3, resulting in the predominant production of 2-monoacylglycerol and free fatty acids. Notably, its enzymatic activity is significantly higher against insoluble emulsified substrates compared to soluble ones. This substrate specificity underscores the protein's efficiency in processing dietary fats and contributing to the digestion and absorption of lipids in the digestive system. PNLIP's functional characteristics highlight its crucial involvement in lipid metabolism, playing a key role in the intricate processes that facilitate the utilization of dietary fats for energy and nutrient absorption.
Technical Parameters
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Species Mouse
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Source E. coli
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Tag N-His;N-SUMO
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Accession
Q6P8U6 (R17-C465)
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Molecular Construction
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N-term
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6*His-SUMO
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PNLIP (R17-C465)
Accession # Q6P8U6 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
PNLIP; Triacylglycerol Lipase; Pancreatic Lipase; PTL; PL; Triacylglycerol Acylhydrolase; Pancreatic Triacylglycerol Lipase; PNLIPD
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AA Sequence
REVCFDKLGCFSDDAPWSGTLDRPLKALPWSPAQINTRFLLYTNENPDNYQLITSDASNIRNSNFRTNRKTRIIIHGFIDKGEENWLSDMCKNMFRVESVNCICVDWKGGSRTTYTQATQNVRVVGAEVALLVNVLQSDLGYSLNNVHLIGHSLGSHIAGEAGKRTFGAIGRITGLDPAEPYFQGTPEEVRLDPTDAQFVDAIHTDAGPIIPNLGFGMSQTVGHLDFFPNGGIEMPGCQKNILSQIVDIDGIWEGTRNFAACNHLRSYKFYTDSIVNPTGFAGFSCSSYSLFTANKCFPCGSGGCPQMGHYADRYPGKTSRLYQTFYLNTGDKSNFARWRYQVTVTLSGQKVTGHILVSLFGNGGNSKQYEVFKGSLQPGTSHVNEFDSDVDVGDLQKVKFIWYNNVINPTLPKVGASRITVERNDGRVFNFCSQETVREDVLLTLSPC
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Predicted Molecular Mass
65.8 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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