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PCDH10 Protein, Human (L405P, HEK293, His)

Cat. No.: HY-P71188
COA Handling Instructions

PCDH10 Protein, a putative calcium-dependent cell-adhesion protein, implies involvement in mediating cell-cell interactions. Its responsiveness to intracellular calcium levels, characteristic of cell-adhesion molecules, suggests roles in cell recognition, adhesion, and signaling. PCDH10's potential as a cell-adhesion protein implies contributions to tissue integrity and cellular regulation across diverse contexts. Further exploration may reveal its molecular interactions and specific roles in cell adhesion, providing insights into physiological processes. PCDH10 Protein, Human (L405P, HEK293, His) is the recombinant human-derived PCDH10 protein, expressed by HEK293 , with C-6*His labeled tag and L405P mutation. The total length of PCDH10 Protein, Human (L405P, HEK293, His) is 697 a.a., with molecular weight of ~87.0 kDa.

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Description

PCDH10 Protein, a putative calcium-dependent cell-adhesion protein, implies involvement in mediating cell-cell interactions. Its responsiveness to intracellular calcium levels, characteristic of cell-adhesion molecules, suggests roles in cell recognition, adhesion, and signaling. PCDH10's potential as a cell-adhesion protein implies contributions to tissue integrity and cellular regulation across diverse contexts. Further exploration may reveal its molecular interactions and specific roles in cell adhesion, providing insights into physiological processes. PCDH10 Protein, Human (L405P, HEK293, His) is the recombinant human-derived PCDH10 protein, expressed by HEK293 , with C-6*His labeled tag and L405P mutation. The total length of PCDH10 Protein, Human (L405P, HEK293, His) is 697 a.a., with molecular weight of ~87.0 kDa.

Background

PCDH10 Protein presents itself as a potential calcium-dependent cell-adhesion protein, suggesting a role in mediating cell-cell interactions. The protein's calcium dependence implies its responsiveness to intracellular calcium levels, which is a characteristic feature of many cell-adhesion molecules involved in processes such as cell recognition, adhesion, and signaling. PCDH10's putative function as a cell-adhesion protein suggests its involvement in maintaining tissue integrity and regulating cellular behavior within diverse biological contexts. Further exploration of its molecular interactions and cellular functions may offer valuable insights into its specific roles in cell adhesion and its potential implications in various physiological processes.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q9P2E7 (Q19-T715, L405P)

Gene ID
Molecular Construction
N-term
PCDH10 (Q19-T715, L405P)
Accession # Q9P2E7
6*His
C-term
Synonyms
Protocadherin-10; PCDH10; KIAA1400
AA Sequence

QLHYTVQEEQEHGTFVGNIAEDLGLDITKLSARGFQTVPNSRTPYLDLNLETGVLYVNEKIDREQICKQSPSCVLHLEVFLENPLELFQVEIEVLDINDNPPSFPEPDLTVEISESATPGTRFPLESAFDPDVGTNSLRDYEITPNSYFSLDVQTQGDGNRFAELVLEKPLDREQQAVHRYVLTAVDGGGGGGVGEGGGGGGGAGLPPQQQRTGTALLTIRVLDSNDNVPAFDQPVYTVSLPENSPPGTLVIQLNATDPDEGQNGEVVYSFSSHISPRARELFGLSPRTGRLEVSGELDYEESPVYQVYVQAKDLGPNAVPAHCKVLVRVLDANDNAPEISFSTVKEAVSEGAAPGTVVALFSVTDRDSEENGQVQCELLGDVPFRPKSSFKNYYTIVTEAPLDREAGDSYTLTVVARDRGEPALSTSKSIQVQVSDVNDNAPRFSQPVYDVYVTENNVPGAYIYAVSATDRDEGANAQLAYSILECQIQGMSVFTYVSINSENGYLYALRSFDYEQLKDFSFQVEARDAGSPQALAGNATVNILIVDQNDNAPAIVAPLPGRNGTPAREVLPRSAEPGYLLTRVAAVDADDGENARLTYSIVRGNEMNLFRMDWRTGELRTARRVPAKRDPQRPYELVIEVRDHGQPPLSSTATLVVQLVDGAVEPQGGGGSGGGGSGEHQRPSRSGGGETSLDLT

Molecular Weight

Approximately 87.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

PCDH10 Protein, Human (L405P, HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PCDH10 Protein, Human (L405P, HEK293, His)
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