PD-L2 Protein, Rhesus Macaque (HEK293, His)
Based on 1 Customer Validation
PDCD1LG2 is an immune checkpoint receptor ligand that binds to PD-1 to activate pathways that inhibit TCR/ BCR-mediated immune cell activation. PDCD1LG2 can inhibit T-cell proliferation and inflammatory cytokine production. The expression of PDCD1LG2 on mouse tumor cells inhibits cytotoxic T-cell-mediated immune responses and can be used as a biomarker or prognostic indicator. PD-L2 Protein, Rhesus Macaque (HEK293, His) is the recombinant cynomolgus-derived PD-L2 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Rhesus Macaque
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
PDCD1LG2 is an immune checkpoint receptor ligand that binds to PD-1 to activate pathways that inhibit TCR/ BCR-mediated immune cell activation. PDCD1LG2 can inhibit T-cell proliferation and inflammatory cytokine production. The expression of PDCD1LG2 on mouse tumor cells inhibits cytotoxic T-cell-mediated immune responses and can be used as a biomarker or prognostic indicator. PD-L2 Protein, Rhesus Macaque (HEK293, His) is the recombinant cynomolgus-derived PD-L2 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
Programmed cell death 1 ligand 2 is a cell surface receptor also known as PD-L2, B7-DC or CD273. PDCD1LG2 is an immune checkpoint receptor ligand that plays a negative regulatory role in adaptive immune response. PDCD1LG2 binds to PD-1 to activate pathways that inhibit TCR/ BCR-mediated immune cell activation. PDCD1LG2 plays an important role in immune tolerance and autoimmunity, and both PD-L1 and PDCD1LG2 can inhibit T cell proliferation and inflammatory cytokine production. Blocking PDCD1LG2 exacerbates experimental autoimmune encephalomyelitis. PDCD1LG2 triggers IL-12 production in mouse dendritic cells, leading to T-cell activation. Treatment with PDCD1LG2 Ig led to a proliferation of T helper cells. The expression of PDCD1LG2 on mouse tumor cells inhibits cytotoxic T cell-mediated immune responses and can be used as a biomarker or prognostic indicator[1][2][3][4][5].
Technical Parameters
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Species Rhesus Macaque
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Source HEK293
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Tag C-6*His
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Accession
A4GW30 (L20-P219)
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Molecular Construction
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N-term
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PD-L2 (L20-P219)
Accession # A4GW30 -
6*His
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C-term
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Protein Length
Partial
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Synonyms
PDCD1LG2; B7 Dendritic Cell Molecule; Programmed Cell Death 1 Ligand 2; Programmed Death Ligand 2; PD-L2; Butyrophilin B7-DC; CD273; PDCD1 Ligand 2; B7-DC; PDCD1L2; PDL2; PD-1-Ligand 2; B7DC; CD273 Antigen; BA574F11.2; PD-1 Ligand 2; Btdc
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AA Sequence
LFTVTVPKELYIIEHGSNATLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLSLGKALFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNISVPANTSHSRTPEGLYQVTSVLRLKPHPGRNFSCVFWNAQVRELTLASIDLQSQIEPRTHP
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Predicted Molecular Mass
23.4 kDa
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Molecular Weight
Approximately 30-40 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Latchman Y, et al. PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol. 2001 Mar;2(3):261-8. [Content Brief]
[2]. Sharpe AH, et al. The function of programmed cell death 1 and its ligands in regulating autoimmunity and infection. Nat Immunol. 2007 Mar;8(3):239-45. [Content Brief]
[3]. Ghiotto M, et al. PD-L1 and PD-L2 differ in their molecular mechanisms of interaction with PD-1. Int Immunol. 2010 Aug;22(8):651-60. [Content Brief]
[4]. Zhang Y, et al. Regulation of T cell activation and tolerance by PDL2. Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11695-700. [Content Brief]
[5]. Tanegashima T, et al. Immune Suppression by PD-L2 against Spontaneous and Treatment-Related Antitumor Immunity. Clin Cancer Res. 2019 Aug 1;25(15):4808-4819. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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