PD-L2 Protein, Cynomolgus (HEK293, C-His)
PD-L2 Protein is an immune checkpoint receptor ligand that binds to PD-1 to activate pathways that inhibit TCR/ BCR-mediated immune cell activation. PDCD1LG2 can inhibit T-cell proliferation and inflammatory cytokine production. The expression of PDCD1LG2 on mouse tumor cells inhibits cytotoxic T-cell-mediated immune responses and can be used as a biomarker or prognostic indicator. PD-L2 Protein, Cynomolgus (HEK293, C-His) is the recombinant cynomolgus-derived PD-L2 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Cynomolgus
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
PD-L2 Protein is an immune checkpoint receptor ligand that binds to PD-1 to activate pathways that inhibit TCR/ BCR-mediated immune cell activation. PDCD1LG2 can inhibit T-cell proliferation and inflammatory cytokine production. The expression of PDCD1LG2 on mouse tumor cells inhibits cytotoxic T-cell-mediated immune responses and can be used as a biomarker or prognostic indicator. PD-L2 Protein, Cynomolgus (HEK293, C-His) is the recombinant cynomolgus-derived PD-L2 protein, expressed by HEK293 , with C-His labeled tag.
Background
Programmed cell death 1 ligand 2 (PDCD1LG2) is a cell surface receptor also known as PD-L2, B7-DC or CD273. PDCD1LG2 is an immune checkpoint receptor ligand that plays a negative regulatory role in adaptive immune response. PDCD1LG2 binds to PD-1 to activate pathways that inhibit TCR/ BCR-mediated immune cell activation. PDCD1LG2 plays an important role in immune tolerance and autoimmunity, and both PD-L1 and PDCD1LG2 can inhibit T cell proliferation and inflammatory cytokine production. Blocking PDCD1LG2 exacerbates experimental autoimmune encephalomyelitis. PDCD1LG2 triggers IL-12 production in mouse dendritic cells, leading to T-cell activation. Treatment with PDCD1LG2 Ig led to a proliferation of T helper cells. The expression of PDCD1LG2 on mouse tumor cells inhibits cytotoxic T cell-mediated immune responses and can be used as a biomarker or prognostic indicator[1][2][3][4].
Technical Parameters
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Species Cynomolgus
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Source HEK293
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Tag C-8*His
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Accession
A0A2K5U5Z4/XP_005581839 (L20-T220)
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Molecular Construction
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N-term
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PD-L2 (L20-T220)
Accession # A0A2K5U5Z4/XP_005581839 -
8*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
PDCD1LG2; B7 Dendritic Cell Molecule; Programmed Cell Death 1 Ligand 2; Programmed Death Ligand 2; PD-L2; Butyrophilin B7-DC; CD273; PDCD1 Ligand 2; B7-DC; PDCD1L2; PDL2; PD-1-Ligand 2; B7DC; CD273 Antigen; BA574F11.2; PD-1 Ligand 2; Btdc
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AA Sequence
LFTVTVPKELYIIEHGSNATLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLSLGKALFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNISVPANTSHSRTPEGLYQVTSVLRLKPHPGRNFSCVFWNAQVRELTLASIDLQSQIEPRTHPT
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Predicted Molecular Mass
23.7 kDa
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Molecular Weight
Approximately 40-50 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
References
[1]. Latchman Y, et al. PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol. 2001 Mar;2(3):261-8. [Content Brief]
[2]. Tseng SY, et al. B7-DC, a new dendritic cell molecule with potent costimulatory properties for T cells. J Exp Med. 2001 Apr 2;193(7):839-46. [Content Brief]
[3]. Wang S, et al. Molecular modeling and functional mapping of B7-H1 and B7-DC uncouple costimulatory function from PD-1 interaction. J Exp Med. 2003 May 5;197(9):1083-91. [Content Brief]
[4]. Masugi Y, et al. Tumor PDCD1LG2 (PD-L2) Expression and the Lymphocytic Reaction to Colorectal Cancer. Cancer Immunol Res. 2017 Nov;5(11):1046-1055. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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