PDGF R beta Protein, Human (HEK293, C-His-Avi)

PDGF Rβ protein is integral in embryonic development and regulates cell proliferation, survival, differentiation, chemotaxis, migration, and vascular development. It crucially recruits pericytes and smooth muscle cells to the endothelium, promoting vascular smooth muscle cell migration and neointimal formation at the site of injury. PDGF R beta Protein, Human (HEK293, C-His-Avi) is the recombinant human-derived PDGF R beta protein, expressed by HEK293 , with C-His, C-Avi labeled tag. The total length of PDGF R beta Protein, Human (HEK293, C-His-Avi) is 498 a.a., with molecular weight of 78-115 kDa.

The receptor described in the text is the platelet-derived growth factor receptor alpha (PDGFRA). PDGFRA is involved in various cellular processes, including embryonic development, cell proliferation, survival, differentiation, chemotaxis, migration, and blood vessel development. It plays a crucial role in the recruitment of pericytes and smooth muscle cells to endothelial cells, as well as in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. PDGFRA is required for normal development of the cardiovascular system and for the formation of a branched network of capillaries in kidney glomeruli. It promotes rearrangement of the actin cytoskeleton and the formation of membrane ruffles. Binding of its ligands, including homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB, or homodimeric PDGFD, leads to the activation of several signaling cascades. PDGFRA phosphorylates various downstream effectors, including PLCG1, PIK3R1, PTPN11, RASA1/GAP, CBL, SHC1, NCK1, PDCD6IP/ALIX, and STAM. The receptor signaling is regulated by protein phosphatases that dephosphorylate the receptor and its downstream effectors, as well as by the rapid internalization of the activated receptor.

Immobilized Human PDGF R beta, His Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-PDGF R beta Antibody, hFc Tag with the EC₅₀ of ≤11 ng/mL determined by ELISA.

Human

HEK293

C-His;C-Avi

P09619 (L33-F530)

5159  [NCBI]

LVVTPPGPELVLNVSSTFVLTCSGSAPVVWERMSQEPPQEMAKAQDGTFSSVLTLTNLTGLDTGEYFCTHNDSRGLETDERKRLYIFVPDPTVGFLPNDAEELFIFLTEITEITIPCRVTDPQLVVTLHEKKGDVALPVPYDHQRGFSGIFEDRSYICKTTIGDREVDSDAYYVYRLQVSSINVSVNAVQTVVRQGENITLMCIVIGNEVVNFEWTYPRKESGRLVEPVTDFLLDMPYHIRSILHIPSAELEDSGTYTCNVTESVNDHQDEKAINITVVESGYVRLLGEVGTLQFAELHRSRTLQVVFEAYPPPTVLWFKDNRTLGDSSAGEIALSTRNVSETRYVSELTLVRVKVAEAGHYTMRAFHEDAEVQLSFQLQINVPVRVLELSESHPDSGEQTVRCRGRGMPQPNIIWSACRDLKRCPRELPPTLLGNSSEEESQLETNVTYWEEEQEFEVVSTLRLQHVDRPLSVRCTLRNAVGQDTQEVIVVPHSLPF

78-115 kDa

Greater than 95% as determined by reducing Tris-Bis PAGE

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.