PDK4 Protein, Mouse (sf9, His-GST)

PDK4 protein is a key kinase that regulates glucose and fatty acid metabolism by phosphorylating pyruvate dehydrogenase subunits PDHA1 and PDHA2.This inhibits pyruvate dehydrogenase activity, modulates metabolite flux and inhibits aerobic respiration.PDK4 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived PDK4 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

PDK4, a pivotal kinase, intricately regulates glucose and fatty acid metabolism by phosphorylating the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This phosphorylation inhibits pyruvate dehydrogenase activity, modulating metabolite flux through the tricarboxylic acid cycle, suppressing aerobic respiration, and impeding the conversion of pyruvate to acetyl-coenzyme A. Consequently, this inhibition decreases glucose utilization and enhances fat metabolism during prolonged fasting and starvation. PDK4's crucial role in maintaining normal blood glucose levels under starvation aligns with its involvement in the insulin signaling cascade. Moreover, it plays a pivotal role in preventing the accumulation of ketone bodies and maintaining normal blood pH. In the fed state, PDK4 mediates cellular responses to glucose levels and high-fat diets, regulating both fatty acid oxidation and de novo fatty acid biosynthesis. Additionally, PDK4 is implicated in the generation of reactive oxygen species and provides protection against anoikis in detached epithelial cells. Its involvement in cell proliferation stems from its regulatory impact on carbohydrate and fatty acid metabolism.

The kinase activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

Sf9 insect cells

N-His;N-GST

O70571 (M1-V412)

27273  [NCBI]

Full Length

MKAARFVMRSASSLSSASLVPREVELFSRYSPSPLSMKQLLDFGSENACERTSFAFLRQELPVRLANILKEIDILPDRLVNTPSVQLVKSWYIQSLMDLVEFHEKSPEDQKALSEFVDTLVKVRNRHHNVVPTMAQGILEYKDTCTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDSKTGNPSHIGSIDPNCDVVAVVQDAFECAKMLCDQYYLTSPELNLTQVNGKFPGQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENRPSLTPVEATVVLGKEDLTIKISDRGGGVPLRITDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNLYSMSGYGTDAIIYLKALSSESVEKLPVFNKSAFKHYQMSSEADDWCIPSREPKNLAKEKLAV

Approximately 65 kDa,based on SDS-PAGE under reducing conditions.

≥ 80%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.