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  4. PDK4 Protein, Mouse (sf9, His-GST)

PDK4 Protein, Mouse (sf9, His-GST)

Cat. No.: HY-P74639
Handling Instructions

PDK4 protein is a key kinase that regulates glucose and fatty acid metabolism by phosphorylating pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, modulates metabolite flux and inhibits aerobic respiration. PDK4 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived PDK4 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of PDK4 Protein, Mouse (sf9, His-GST) is 412 a.a., with molecular weight of ~65 kDa.

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Description

PDK4 protein is a key kinase that regulates glucose and fatty acid metabolism by phosphorylating pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, modulates metabolite flux and inhibits aerobic respiration. PDK4 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived PDK4 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of PDK4 Protein, Mouse (sf9, His-GST) is 412 a.a., with molecular weight of ~65 kDa.

Background

PDK4, a pivotal kinase, intricately regulates glucose and fatty acid metabolism by phosphorylating the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This phosphorylation inhibits pyruvate dehydrogenase activity, modulating metabolite flux through the tricarboxylic acid cycle, suppressing aerobic respiration, and impeding the conversion of pyruvate to acetyl-coenzyme A. Consequently, this inhibition decreases glucose utilization and enhances fat metabolism during prolonged fasting and starvation. PDK4's crucial role in maintaining normal blood glucose levels under starvation aligns with its involvement in the insulin signaling cascade. Moreover, it plays a pivotal role in preventing the accumulation of ketone bodies and maintaining normal blood pH. In the fed state, PDK4 mediates cellular responses to glucose levels and high-fat diets, regulating both fatty acid oxidation and de novo fatty acid biosynthesis. Additionally, PDK4 is implicated in the generation of reactive oxygen species and provides protection against anoikis in detached epithelial cells. Its involvement in cell proliferation stems from its regulatory impact on carbohydrate and fatty acid metabolism.

Species

Mouse

Source

Sf9 insect cells

Tag

N-His;N-GST

Accession

O70571 (M1-V412)

Gene ID

27273  [NCBI]

Molecular Construction
N-term
His-GST
PDK4 (M1-V412)
Accession # O70571
C-term
Synonyms
Pyruvate dehydrogenase kinase isoform 4; PDK4; PDHK4
Molecular Weight

Approximately 65 kDa

Purity

Greater than 85% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

PDK4 Protein, Mouse (sf9, His-GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PDK4 Protein, Mouse (sf9, His-GST)
Cat. No.:
HY-P74639
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