Periostin Protein, Human (815a.a, HEK293, His)
Based on 1 Customer Validation
Periostin protein, a multifaceted regulator, induces cell attachment and spreading, participates in cell adhesion, and enhances BMP1 incorporation into the fibronectin matrix. It forms a structural homodimer and interacts with BMP1 and fibronectin. This interplay positions Periostin as a key player in regulating cellular and tissue functions, including cell adhesion, tissue architecture, and extracellular matrix modulation. Periostin Protein, Human (815a.a, HEK293, His) is the recombinant human-derived Periostin protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Periostin protein, a multifaceted regulator, induces cell attachment and spreading, participates in cell adhesion, and enhances BMP1 incorporation into the fibronectin matrix. It forms a structural homodimer and interacts with BMP1 and fibronectin. This interplay positions Periostin as a key player in regulating cellular and tissue functions, including cell adhesion, tissue architecture, and extracellular matrix modulation. Periostin Protein, Human (815a.a, HEK293, His) is the recombinant human-derived Periostin protein, expressed by HEK293 , with C-His labeled tag.
Background
Periostin protein emerges as a multifaceted regulator, demonstrating its capability to induce cell attachment and spreading while actively participating in cell adhesion. Additionally, Periostin enhances the incorporation of BMP1 into the fibronectin matrix within connective tissues, subsequently facilitating the proteolytic activation of lysyl oxidase LOX. This multifunctional protein exists as a homodimer, emphasizing its structural significance. Notably, Periostin engages in interactions with BMP1 and fibronectin, underscoring its involvement in pivotal molecular processes that contribute to cell adhesion, tissue architecture, and extracellular matrix modulation. The intricate interplay between Periostin and its binding partners positions it as a key player in the dynamic regulation of cellular and tissue functions.
Verified Bioactivity
1.Immobilized Human Periostin, His Tag at 1 μg/mL (100 μL/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 3.9-9.3 ng/mL determined by ELISA.
2.Immobilized Human Periostin, His Tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of ≤18.5 ng/mL determined by ELISA.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-8*His
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Accession
Q15063-1 (N22-Q836)
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Molecular Construction
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N-term
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Periostin (N22-Q836)
Accession # Q15063-1 -
8*His
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C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
POSTN; Osteoblast Specific Factor 2 (Fasciclin I-Like); Periostin; Periodontal Ligament-Specific Periostin; OSF-2; Osteoblast Specific Factor 2; PN; Osteoblast-Specific Factor 2; Periostin, Osteoblast Specific Factor; PDLPOSTN; OSF2
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AA Sequence
NNHYDKILAHSRIRGRDQGPNVCALQQILGTKKKYFSTCKNWYKKSICGQKTTVLYECCPGYMRMEGMKGCPAVLPIDHVYGTLGIVGATTTQRYSDASKLREEIEGKGSFTYFAPSNEAWDNLDSDIRRGLESNVNVELLNALHSHMINKRMLTKDLKNGMIIPSMYNNLGLFINHYPNGVVTVNCARIIHGNQIATNGVVHVIDRVLTQIGTSIQDFIEAEDDLSSFRAAAITSDILEALGRDGHFTLFAPTNEAFEKLPRGVLERIMGDKVASEALMKYHILNTLQCSESIMGGAVFETLEGNTIEIGCDGDSITVNGIKMVNKKDIVTNNGVIHLIDQVLIPDSAKQVIELAGKQQTTFTDLVAQLGLASALRPDGEYTLLAPVNNAFSDDTLSMDQRLLKLILQNHILKVKVGLNELYNGQILETIGGKQLRVFVYRTAVCIENSCMEKGSKQGRNGAIHIFREIIKPAEKSLHEKLKQDKRFSTFLSLLEAADLKELLTQPGDWTLFVPTNDAFKGMTSEEKEILIRDKNALQNIILYHLTPGVFIGKGFEPGVTNILKTTQGSKIFLKEVNDTLLVNELKSKESDIMTTNGVIHVVDKLLYPADTPVGNDQLLEILNKLIKYIQIKFVRGSTFKEIPVTVYTTKIITKVVEPKIKVIEGSLQPIIKTEGPTLTKVKIEGEPEFRLIKEGETITEVIHGEPIIKKYTKIIDGVPVEITEKETREERIITGPEIKYTRISTGGGETEETLKKLLQEEVTKVTKFIEGGDGHLFEDEEIKRLLQGDTPVRKLQANKKVQGSRRRLREGRSQ
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Predicted Molecular Mass
92.1 kDa
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Molecular Weight
Approximately 93-100 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (241 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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