BAR/Phosphinothricin N-acetyltransferase Protein, Streptomyces hygroscopicus

The BAR/glufosinate N-acetyltransferase protein plays a key role in inactivating glufosinate (PPT) by promoting acetyl transfer of acetyl-CoA. BAR/Phosphinothricin N-acetyltransferase Protein, Streptomyces hygroscopicus is the recombinant BAR/Phosphinothricin N-acetyltransferase protein, expressed by E. coli , with tag free.

The BAR/Phosphinothricin N-acetyltransferase protein plays a pivotal role in inactivating phosphinothricin (PPT) by facilitating the transfer of an acetyl group from acetyl CoA. Notably, it exhibits the ability to acetylate demethylphosphinothricin while remaining inactive toward PTT or glutamate. This enzymatic specificity positions BAR as a key effector in conferring resistance to phosphinothricin tripeptide (PTT or bialaphos), highlighting its functional significance in cellular responses to these compounds.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Others

E. coli

Tag Free

P16426 (M1-I183)

/

Full Length

MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQEWTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGSTLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGFWQLDFSLPVPPRPVLPVTEI

18-20 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 12.5 mM Tris-HCl, 50 mM NaCl, ‎‎5% trehalose, 5% mannitol, 0.01% Tween 80, 2 mM DTT, 1 mM EDTA, pH 8.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.